Description

Glycerol Dehydrogenase is a highly specific oxidoreductase enzyme that catalyzes the reversible oxidation of glycerol to dihydroxyacetone. This reaction is fundamental in biochemical pathways for glycerol metabolism and bioanalytical applications. It is an essential reagent for enzymatic assays in life science research, clinical diagnostics, and industrial biocatalysis. Key users include manufacturers of diagnostic kits, biochemical research laboratories, and companies developing bio-based chemical processes.

Application

• Clinical & Diagnostic Assays: Core component in enzymatic test kits for the quantitative determination of glycerol, triglycerides, and lipase activity in serum and other biological fluids.
• Biochemical Research: Used as a model enzyme in studies of dehydrogenase kinetics, enzyme mechanisms, and cofactor (NAD+/NADH) recycling systems.
• Industrial Biocatalysis: Employed in the synthesis of fine chemicals and pharmaceutical intermediates, particularly for the stereoselective oxidation or reduction of glycerol derivatives.
• Food & Beverage Analysis: Applied in enzymatic methods for the accurate measurement of glycerol content in wine, beer, and other fermented products for quality control.
• Biofuel & Biodiesel Research: Utilized in enzymatic pathways for the conversion of glycerol, a major byproduct of biodiesel production, into higher-value chemicals.
• Environmental Monitoring: Incorporated into biosensor platforms for detecting glycerol and related compounds in environmental samples.

Basic Information

Product Name	Glycerol Dehydrogenase
CAS No.	9028-14-2
Molecular Formula	N/A (Enzyme)
Molecular Weight	~78 kDa (Subunit composition dependent)
Synonyms	Glycerol:NAD+ 2-oxidoreductase; GDH; Glycerol Dehydrogenase (NAD+); 1,2-Propanediol Dehydrogenase; Glycerol Dehydrogenase from *Cellulomonas* sp.; NAD+-Linked Glycerol Dehydrogenase; Glycerol Oxidoreductase; EC 1.1.1.6
EINECS	232-848-1

Quality Control

Our Glycerol Dehydrogenase is produced and tested under stringent quality management systems. Each lot is characterized for critical parameters including specific activity, purity, and stability to ensure consistent performance in sensitive applications. A comprehensive Certificate of Analysis (COA) detailing assay results, protein content, and storage buffer information is provided with every shipment to guarantee traceability and reliability for your research and production needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C. Reconstituted enzyme solutions should be aliquoted and stored at -20°C or below to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Activity)	Catalyzes the oxidation of glycerol with NAD+
Specific Activity	≥ 50 units/mg protein
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	≥ 90%
Stabilizers	Potassium phosphate, EDTA
pH (in solution)	7.0 - 7.5
Storage Buffer	50 mM Potassium Phosphate

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.