Description

Adenylosuccinate Lyase is a crucial enzyme in the purine nucleotide biosynthetic pathway, specifically catalyzing the cleavage of adenylosuccinate to form adenosine monophosphate (AMP) and fumarate. This reaction is vital for cellular energy metabolism and nucleotide recycling, making the enzyme an essential reagent for biochemical research and diagnostic applications. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in metabolic disorder studies and enzyme replacement therapy development.

Application

• Biochemical Research: Essential for in vitro studies of the purine biosynthesis and salvage pathways, enzyme kinetics, and metabolic flux analysis.
• Diagnostic Assay Development: Used as a key component in enzymatic assays for the diagnosis of adenylosuccinate lyase deficiency (ADSL), a rare neurometabolic disorder.
• Pharmaceutical Screening: Serves as a target enzyme for high-throughput screening of potential inhibitors or modulators for therapeutic intervention.
• Enzyme Standardization: Acts as a reference standard for calibrating diagnostic equipment and validating assay protocols in clinical laboratories.
• Educational Kits: Incorporated into teaching kits for university-level biochemistry courses to demonstrate enzyme function and metabolic pathways.
• Biocatalyst Development: Investigated for potential use in synthetic biology applications and the enzymatic synthesis of nucleotide analogs.

Basic Information

Product Name	Adenylosuccinate Lyase
CAS No.	9027-81-0
Molecular Formula	N/A (Enzyme)
Molecular Weight	~55 kDa (monomer, species-dependent)
Synonyms	ADSL; Adenylosuccinase; 6-N-(1,2-Dicarboxyethyl)-AMP AMP-lyase (fumarate-forming); SAICAR lyase; IMP--aspartate ligase; EC 4.3.2.2; Adenylosuccinate lyase (ADSL)
EINECS	Contact for details

Quality Control

Our Adenylosuccinate Lyase is produced and tested under stringent quality management systems. Each lot undergoes comprehensive analysis to ensure high specific activity, purity, and stability. Key quality attributes verified include protein concentration (via UV absorbance), specific activity (measured by continuous spectrophotometric assay), and purity assessment (via SDS-PAGE and HPLC). A Certificate of Analysis (COA) detailing batch-specific results is provided with every shipment to guarantee traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C or below. Reconstituted enzyme solutions should be aliquoted and stored at -70°C to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly in a dry environment after each use.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Activity)	Catalyzes the cleavage of adenylosuccinate
Protein Content (Biuret)	> 90%
Specific Activity	> 10 units/mg protein
Purity (SDS-PAGE)	> 95%
Solubility	Soluble in buffer or water
pH (1% solution)	6.0 - 8.0
Endotoxin (LAL)	< 1.0 EU/μg (if required)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.