Description

n-Acetylneuraminic Acid Aldolase is a key biocatalyst enzyme that cleaves N-acetylneuraminic acid (Neu5Ac) into N-acetylmannosamine and pyruvate. This reaction is fundamental in the biosynthesis and metabolic research of sialic acids, which are critical components in glycobiology and cellular recognition processes. It is an essential reagent for researchers and manufacturers in the pharmaceutical, diagnostic, and life science sectors, particularly for the enzymatic synthesis of sialic acid derivatives and related carbohydrate-based compounds.

Application

• Enzymatic Synthesis: Key catalyst for the production of sialic acid (N-acetylneuraminic acid) and its derivatives from precursor molecules.
• Glycobiology Research: Used to study sialic acid metabolism, catabolism, and the function of sialylated glycoconjugates in cell signaling and pathogen recognition.
• Diagnostic Reagent Production: Employed in the manufacturing of enzymatic assay kits for the detection and quantification of sialic acids in clinical and research samples.
• Biopharmaceutical Development: Applied in the research and development of glycoprotein therapeutics, vaccines, and anti-inflammatory drugs where sialylation patterns are crucial.
• Biocatalysis & Process Chemistry: Utilized in green chemistry approaches for the stereoselective synthesis of complex carbohydrates under mild conditions.
• Food & Nutraceutical Analysis: Assists in analyzing sialic acid content in functional foods, infant formula, and dietary supplements.

Basic Information

Product Name	n-Acetylneuraminic Acid Aldolase
CAS No.	9027-60-5
Molecular Formula	N/A (Enzyme)
Molecular Weight	Approx. 32-38 kDa (monomer) - Contact for details
Synonyms	Neu5Ac Aldolase; Sialic Acid Aldolase; N-Acetylneuraminate Lyase; N-Acetylneuraminic Acid Lyase; Neuraminic Acid Aldolase; Aldolase, N-Acetylneuraminate; EC 4.1.3.20; NAL; Sialate Pyruvate-Lyase
EINECS	Contact for details

Quality Control

Our n-Acetylneuraminic Acid Aldolase is produced and tested under stringent quality management systems. Each batch is characterized for specific activity, purity, and stability to ensure consistent performance. Certificates of Analysis (COA) are provided, detailing parameters such as protein concentration, specific activity (U/mg), and absence of contaminating enzymatic activities. We support compliance with relevant guidelines for research and manufacturing use.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C or below. Reconstituted enzyme solutions should be aliquoted and stored at -20°C to -80°C to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use in a low-humidity environment.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Activity)	Catalyzes the cleavage of Neu5Ac
Protein Content (Biuret)	>90%
Specific Activity	>1.0 U/mg protein
Purity (SDS-PAGE)	Single major band
pH Optimum	7.0 - 7.5
Temperature Optimum	37°C
Stability	>90% activity after 12 months at -20°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.