Description

β-n-Acetyl-D-Hexosaminidase is a crucial lysosomal enzyme belonging to the glycosyl hydrolase family. This enzyme is essential for the catabolic breakdown of glycosphingolipids, specifically cleaving terminal β-linked N-acetylglucosamine and N-acetylgalactosamine residues. It is a vital reagent for biochemical research, diagnostic assay development, and the study of metabolic disorders. Key industries that rely on this high-purity enzyme include pharmaceutical research, in-vitro diagnostics, and academic life sciences.

Application

• Biochemical Research & Enzyme Kinetics: Used as a standard enzyme in studies of glycosidase activity, inhibition, and mechanism of action.
• Diagnostic Assay Development: Serves as a critical component in the development of enzymatic assays for clinical diagnostics, particularly for lysosomal storage disorders like Tay-Sachs and Sandhoff diseases.
• Substrate Specificity Studies: Employed to investigate the activity and specificity on various natural and synthetic glycoside substrates.
• Pharmaceutical Screening: Utilized in high-throughput screening platforms to identify potential therapeutic inhibitors or activators for drug discovery.
• Quality Control Reference Standard: Acts as a reference material for the calibration and validation of diagnostic kits and laboratory tests.
• Glycobiology & Glycoprotein Analysis: Applied in the structural analysis of glycoproteins and oligosaccharides by selectively cleaving specific sugar linkages.

Basic Information

Product Name	β-n-Acetyl-D-Hexosaminidase
CAS No.	9027-52-5
Molecular Formula	N/A (Enzyme)
Molecular Weight	Varies by isoform and source (typically ~100-120 kDa for dimeric forms)
Synonyms	N-Acetyl-β-D-hexosaminidase; N-Acetyl-β-glucosaminidase; β-Hexosaminidase; Hexosaminidase A & B; NAGase; EC 3.2.1.52; β-N-acetylhexosaminidase; 2-Acetamido-2-deoxy-β-D-glucoside acetamidodeoxyglucohydrolase
EINECS	Contact for details

Quality Control

Our β-n-Acetyl-D-Hexosaminidase is produced and tested under stringent quality management systems. Each lot undergoes rigorous analytical testing to ensure high specific activity, purity, and stability. Quality parameters typically assessed include specific activity (U/mg protein), protein content, and absence of contaminating protease activities. A comprehensive Certificate of Analysis (COA) detailing batch-specific results is provided with every shipment to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C or below. For solution forms, aliquot and store at -20°C to -80°C to avoid repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly in a dry environment after use.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Activity)	Conforms (Hydrolyzes specific synthetic substrate)
Specific Activity	> 50 U/mg protein (Contact for lot-specific data)
Protein Content (Bradford)	> 90%
Purity (SDS-PAGE)	Single major band
Solubility	Soluble in water or appropriate buffer
pH Optimum	~4.0 - 4.5 (varies by substrate and source)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.