Description

Pyruvate, Orthophosphate Dikinase CAS NO 9027-40-1 is a critical enzyme that catalyzes the reversible conversion of pyruvate, phosphate, and ATP into phosphoenolpyruvate, AMP, and pyrophosphate. This reaction is fundamental to gluconeogenesis and the regulation of carbon metabolism in plants and certain microorganisms. It is an essential reagent for biochemical research, metabolic pathway analysis, and the development of enzymatic assays. Industrial and academic researchers in life sciences, agricultural biotechnology, and biofuel production rely on this high-purity enzyme for consistent and reproducible results.

Application

• Biochemical Research & Metabolic Studies: A key reagent for investigating gluconeogenesis, the Calvin cycle, and C4 plant photosynthesis pathways.
• Enzymatic Assay Development: Used in the design and validation of diagnostic and research kits for measuring related metabolites or enzyme activities.
• Plant Physiology & Agricultural Biotechnology: Critical for studies aimed at enhancing crop yield and stress tolerance by manipulating photosynthetic efficiency.
• Biofuel & Industrial Biotechnology: Employed in metabolic engineering of microorganisms for the production of biofuels and biochemicals from renewable resources.
• Pharmaceutical Research: Potential target for herbicide development and research into metabolic disorders.
• Educational & Diagnostic Use: Serves as a standard enzyme in university laboratories and for quality control in related diagnostic manufacturing.

Basic Information

Product Name	Pyruvate, Orthophosphate Dikinase
CAS No.	9027-40-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Pyruvate, Phosphate Dikinase; PPDK; Pyruvate Orthophosphate Dikinase; ATP:pyruvate, orthophosphate phosphotransferase; Pyruvate,Pi Dikinase; EC 2.7.9.1; Phosphoenolpyruvate synthase (pyruvate phosphorylation); PEP synthase
EINECS	Contact for details

Quality Control

Our Pyruvate, Orthophosphate Dikinase is produced and tested under stringent quality management protocols to ensure high specific activity, purity, and batch-to-batch consistency suitable for research applications. Each lot is characterized for activity (U/mg) and purity profile. A comprehensive Certificate of Analysis (COA) detailing specific activity, purity, and stability data is provided with every shipment to support your quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation. Aliquot reconstituted enzyme to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Activity)	Conforms
Specific Activity	≥ [Value] units/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Content (Biuret)	Reported on COA
Solubility	Clear, colorless solution in specified buffer
Stability	Stable as lyophilized powder at recommended storage

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.