Description

3-Deoxy-D-Manno-Octulosonate Aldolase is a highly specific enzyme that catalyzes the reversible aldol cleavage of 3-deoxy-D-manno-octulosonate (KDO). This biocatalyst is essential for research and development in the field of carbohydrate metabolism and bacterial cell wall biosynthesis. It is a critical reagent for biochemical research, pharmaceutical development, and enzymatic synthesis, particularly for scientists investigating gram-negative bacterial pathways and glycan synthesis.

Application

• Biochemical Research: A key tool for studying the KDO pathway, bacterial lipopolysaccharide (LPS) biosynthesis, and carbohydrate metabolism.
• Pharmaceutical Development: Used in target validation and inhibitor screening for novel antibiotics targeting gram-negative bacteria.
• Enzymatic Synthesis: Employed in the synthesis of complex carbohydrates, oligosaccharides, and KDO derivatives for research purposes.
• Diagnostic Reagent Manufacturing: Serves as a component in enzymatic assay kits for research and diagnostic applications related to bacterial metabolism.
• Academic & Industrial R&D: Essential for laboratories in microbiology, glycobiology, and medicinal chemistry for pathway analysis and compound characterization.

Basic Information

Product Name	3-Deoxy-D-Manno-Octulosonate Aldolase
CAS No.	9026-95-3
Molecular Formula	N/A (Enzyme)
Molecular Weight	Contact for details
Synonyms	KDO Aldolase; 3-Deoxy-D-manno-octulosonate 8-phosphate aldolase; 2-Dehydro-3-deoxy-D-octonate 8-phosphate aldolase; KDO-8-P aldolase; EC 4.1.2.16; 3-Deoxy-D-manno-octulosonic acid aldolase; KDO synthase (ambiguous)
EINECS	Contact for details

Quality Control

Our 3-Deoxy-D-Manno-Octulosonate Aldolase is produced and tested under stringent conditions to ensure high activity, purity, and batch-to-batch consistency. Each lot is characterized for specific activity and protein content. A comprehensive Certificate of Analysis (COA) is provided, detailing purity, activity (U/mg), and stability data, supporting research reproducibility and compliance with good laboratory practice (GLP).

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. Aliquot reconstituted solutions to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder
Protein Content (Biuret)	> 90%
Specific Activity	> 10 units/mg protein
Purity (SDS-PAGE)	Single major band
Stabilizers	Potassium phosphate, DTT
Solubility	Soluble in aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.