Description

Nuclease is a hydrolytic enzyme that catalyzes the cleavage of phosphodiester bonds in nucleic acids (DNA and RNA). This product is essential for applications requiring the removal or digestion of nucleic acids to prevent contamination or interference in downstream processes. It is a critical reagent for molecular biology research, pharmaceutical manufacturing, and bioprocessing industries.

Application

• Pharmaceutical Manufacturing: Removal of host cell DNA/RNA from recombinant protein and vaccine preparations to meet regulatory purity standards.
• Molecular Biology Research: DNA/RNA digestion in sample preparation, nucleic acid extraction protocols, and elimination of nucleic acid contamination from enzyme preparations.
• Bioprocessing: Degradation of nucleic acids in cell lysates to reduce viscosity and improve the efficiency of downstream filtration and purification steps.
• Diagnostic Kit Production: Used as a component in diagnostic kits and reagents to ensure sample integrity by degrading unwanted nucleic acids.
• Food & Beverage Analysis: Applied in analytical methods to break down nucleic acids from microbial sources for quality control and safety testing.

Basic Information

Product Name	Nuclease
CAS No.	9026-81-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Nuclease S7; Micrococcal Nuclease; Staphylococcal Nuclease; Endonuclease (Micrococcal); EC 3.1.31.1; Benzonase® (alternative trade name for engineered nuclease); Deoxyribonuclease (non-specific); Phosphodiesterase
EINECS	Contact for details

Quality Control

Our Nuclease is produced and tested under a rigorous quality management system. Each batch is analyzed to ensure high specific activity, purity, and the absence of contaminating protease or phosphatase activities. A comprehensive Certificate of Analysis (COA) detailing assay results, purity, and stability is provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive). Allow the vial to reach room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to standard
Assay (Activity)	≥ 15,000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
Protease Activity	Not detected
Phosphatase Activity	Not detected
pH (in solution)	7.0 - 8.0
Endotoxin	< 10 EU/mg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.