Description

Ribonuclease T1 is a highly specific endoribonuclease that catalyzes the cleavage of single-stranded RNA at guanosine residues. This enzyme is a critical tool for RNA sequence analysis, mapping, and structural studies, offering exceptional specificity and reliability. It is essential for molecular biology research, pharmaceutical development, and diagnostic applications where precise RNA manipulation is required.

Application

• RNA Sequencing and Fingerprinting: Used for enzymatic RNA sequencing and generating specific oligonucleotide fragments for analysis.
• Structural Biology Studies: Employed as a probe for RNA secondary and tertiary structure determination.
• Pharmaceutical R&D: A key reagent in the development of RNA-based therapeutics, including mRNA vaccines and antisense oligonucleotides.
• Diagnostic Kit Manufacturing: Incorporated into kits for the detection and analysis of viral RNA and other nucleic acid targets.
• Biochemical Research: Essential for studying RNA-protein interactions, ribozyme activity, and RNA metabolism pathways.
• Quality Control in Biotechnology: Used to assess the integrity and purity of RNA samples in production and research settings.

Basic Information

Product Name	Ribonuclease T1
CAS No.	9026-12-4
Molecular Formula	N/A (Protein)
Molecular Weight	~11,000 Da (Monomer)
Synonyms	RNase T1; Guanyloribonuclease; Ribonuclease Guanine-specific; Endoribonuclease T1; Aspergillus oryzae Ribonuclease; EC 3.1.27.3; Ribonucleate guaninenucleotide-2'-transferase (cyclizing)
EINECS	232-846-5

Quality Control

Our Ribonuclease T1 is produced and tested under stringent quality management systems. Each batch is subjected to comprehensive analytical testing to ensure high purity, specific activity, and functional integrity. Certificates of Analysis (COA) detailing purity, activity units, and absence of contaminating nucleases are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to standard
Specific Activity	≥ 200,000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
Protein Content (Biuret)	≥ 90%
DNase Activity	Not detectable
Phosphodiesterase Activity	Not detectable
Solubility	Clear, colorless solution in water or buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.