Description

3-Hydroxyisobutyryl-Coa Hydrolase is a specialized enzyme critical for catalyzing the hydrolysis of 3-hydroxyisobutyryl-CoA, a key intermediate in the valine and odd-chain fatty acid catabolic pathways. This enzyme matters for researchers and industrial biochemists seeking to modulate metabolic flux, study inborn errors of metabolism, or develop biocatalytic processes. It is primarily needed by biotechnology firms, academic research institutions, and pharmaceutical companies engaged in metabolic engineering, diagnostic assay development, and fundamental biochemical research.

Application

• Metabolic Pathway Research: Essential for in vitro studies of valine catabolism and odd-chain fatty acid degradation.
• Enzyme Deficiency Studies: Used as a reference standard in research related to metabolic disorders such as 3-hydroxyisobutyric aciduria.
• Biocatalyst Development: Potential application in synthetic biology and green chemistry for the stereoselective synthesis of chiral intermediates.
• Diagnostic Kit Manufacturing: A key component in enzymatic assays for clinical diagnostics and newborn screening programs.
• Pharmaceutical R&D: Employed in drug discovery projects targeting metabolic enzymes and pathways.
• Biochemical Reagent: Serves as a high-purity reagent for enzymatic activity assays and kinetic studies in academic and industrial laboratories.

Basic Information

Product Name	3-Hydroxyisobutyryl-Coa Hydrolase
CAS No.	9025-88-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	3-Hydroxyisobutyryl-CoA Hydrolase; 3HIB-CoA Hydrolase; EC 3.1.2.-; Methylmalonate semialdehyde dehydrogenase (acylating); MMSDH; 3-Hydroxyisobutyryl-Coenzyme A Hydrolase; Valine Catabolism Enzyme; 3-Hydroxy-2-methylpropionyl-CoA hydrolase
EINECS	Contact for details

Quality Control

Our 3-Hydroxyisobutyryl-Coa Hydrolase is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and high enzymatic activity. Each lot is characterized for specific activity, purity, and stability. Certificates of Analysis (COA) detailing assay results, protein content, and storage buffer information are available upon request to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. Avoid repeated freeze-thaw cycles. This material is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent moisture absorption, which can affect activity.

Specification

Item	Specification
Appearance	Lyophilized powder or clear solution
Identification (IR)	Conforms
Protein Content (Biuret)	>90%
Specific Activity	≥ XX units/mg protein
Purity (SDS-PAGE)	≥ 95%
pH (in storage buffer)	7.0 - 7.5
Endotoxin (LAL)	< 1.0 EU/µg (Research Grade)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.