Description

β-Mannosidase is a hydrolytic enzyme that specifically cleaves terminal, non-reducing β-D-mannose residues in glycans and glycoconjugates. This precise catalytic activity is essential for the structural analysis and modification of complex carbohydrates, including N-linked glycans and mannans. It is a critical reagent for biotechnology, pharmaceutical, and life science research sectors, particularly in glycomics, glycoprotein characterization, and the development of enzyme-based diagnostic assays.

Application

• Glycan Structure Analysis: Used in sequencing and profiling of N-linked and O-linked glycans from therapeutic proteins and antibodies.
• Biopharmaceutical Development: Employed for the characterization and quality control of glycoprotein-based drugs, including monoclonal antibodies and recombinant hormones.
• Enzymatic Synthesis: Acts as a tool in the chemo-enzymatic synthesis of defined oligosaccharides and glycoconjugates for research and diagnostic use.
• Biomass Conversion Research: Applied in studies of hemicellulose (e.g., mannan) degradation for biofuel and renewable chemical production.
• Diagnostic Reagent Manufacturing: Incorporated into enzyme kits for the detection of specific genetic disorders related to lysosomal storage, such as β-mannosidosis.
• Food & Feed Industry Research: Investigated for its potential to modify non-starch polysaccharides to improve nutritional value and digestibility.

Basic Information

Product Name	β-Mannosidase
CAS No.	9025-43-8
Molecular Formula	N/A (Enzyme)
Molecular Weight	Varies by source and preparation
Synonyms	β-Mannosidase; β-D-Mannosidase; Mannanase (specific); Exo-β-mannanase; β-Mannoside mannohydrolase; Lysosomal β-A-mannosidase; MANBA; EC 3.2.1.25
EINECS	Contact for details

Quality Control

Our β-Mannosidase is produced and tested under stringent quality management systems. Each lot is characterized for specific activity, purity, and absence of interfering enzymatic activities. Certificates of Analysis (COA) detailing assay results, protein content, and stability data are provided to ensure it meets the rigorous standards required for research and bioprocessing applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Specific Activity	> X U/mg protein (Grade dependent)
Purity (SDS-PAGE)	> 90%
Protein Content (Bradford)	Reported on COA
pH Optimum	~4.0 - 5.0 (Source dependent)
Temperature Optimum	~37°C - 50°C
Stabilizers / Buffers	May contain glycerol or buffer salts
Endotoxin Level	< 1.0 EU/μg (For injectable grade)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.