Description

Heparinase I is a highly specific lyase enzyme that cleaves heparin and heparan sulfate glycosaminoglycans at glucosamine-uronic acid linkages. This precise enzymatic activity is critical for the structural analysis and depolymerization of these complex polysaccharides. It is an essential tool for researchers and manufacturers in the pharmaceutical and biotechnology sectors, particularly for developing heparin-based therapeutics and diagnostics.

Application

• Pharmaceutical Analysis & Quality Control: Used for the structural characterization, sequencing, and purity assessment of heparin and low-molecular-weight heparin (LMWH) APIs to ensure batch consistency and regulatory compliance.
• Heparin Depolymerization: Employed in the controlled production of ultra-low-molecular-weight heparin (ULMWH) fragments and oligosaccharides for novel anticoagulant drug development.
• Biomedical Research: Essential for studying the role of heparan sulfate proteoglycans in biological processes such as cell signaling, angiogenesis, viral entry, and cancer metastasis.
• Diagnostic Kit Manufacturing: Incorporated into in-vitro diagnostic (IVD) kits and assays designed to measure heparin levels or activity in clinical blood samples.
• Bioprocessing: Applied to remove heparin from biological samples or cell cultures to purify other components or to study heparin-independent mechanisms.
• Glycobiology & Biochemistry: A fundamental reagent for enzymatic mapping and determining the fine structure of complex glycosaminoglycan (GAG) chains.

Basic Information

Product Name	Heparinase I
CAS No.	9025-39-2
Molecular Formula	N/A (Enzyme)
Molecular Weight	~42.8 kDa (Monomer)
Synonyms	Heparin Lyase I; Heparinase I from Flavobacterium heparinum; Heparin eliminase; EC 4.2.2.7; Heparinase; Flavobacterium heparinase I; Heparin degrading enzyme
EINECS	Contact for details

Quality Control

Our Heparinase I is produced and tested under stringent quality management systems. Each lot is characterized for specific activity, purity, and absence of contaminating activities (e.g., Heparinase II/III, proteases, nucleases). A comprehensive Certificate of Analysis (COA) detailing assay results, storage buffer, and unit definition is provided with every shipment to ensure reproducibility and reliability for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C or below. Reconstituted enzyme solutions should be aliquoted and stored at -70°C to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive).

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Activity)	Cleaves heparin to characteristic unsaturated disaccharides
Specific Activity	≥ 30,000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
Contaminating Activities	Heparinase II & III activity not detectable
Protein Content (Bradford)	Contact for details
Solubility	Soluble in water or appropriate aqueous buffer
Storage Buffer (Lyophilized)	Typically Tris or phosphate buffer salts

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.