Description

Cytosine Deaminase is a crucial enzyme that catalyzes the hydrolytic deamination of cytosine to uracil. This biochemical activity is fundamental in nucleotide metabolism and has significant value in targeted cancer therapy and molecular biology research. It is primarily utilized by pharmaceutical R&D teams, biotechnology firms, and academic research institutions developing enzyme-prodrug systems and studying pyrimidine salvage pathways.

Application

• Enzyme-Prodrug Therapy (EPT): A key component in gene-directed enzyme-prodrug therapy (GDEPT) strategies, particularly for converting the prodrug 5-fluorocytosine (5-FC) into the potent anticancer agent 5-fluorouracil (5-FU) within tumor cells.
• Cancer Research: Used in experimental models to study and develop novel targeted treatments for various cancers, including colorectal, brain, and breast tumors.
• Molecular Biology: Employed as a tool enzyme in genetic studies, metabolic pathway analysis, and the investigation of nucleotide metabolism and DNA repair mechanisms.
• Biocatalysis & Synthesis: Applied in the enzymatic synthesis of nucleoside analogs and other fine chemicals, offering a selective and environmentally friendly alternative to traditional chemical methods.
• Diagnostic Development: Potential use in the development of biosensors and diagnostic assays for detecting specific genetic markers or enzymatic activities.
• Microbiological Studies: Used in research on microbial metabolism, as the enzyme is present in certain bacteria and fungi, playing a role in their pyrimidine utilization pathways.

Basic Information

Product Name	Cytosine Deaminase
CAS No.	9025-05-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cytosine Aminohydrolase; CDase; CD; Cytosine Deaminase (E.C. 3.5.4.1); Cytosine Deaminase from E. coli; Cytosine Deaminase from Yeast; 4-Aminopyrimidin-2(1H)-one Deaminase; Cytosine Deaminase Enzyme
EINECS	Contact for details

Quality Control

Our Cytosine Deaminase is produced and tested under stringent quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, specific activity, and functional consistency. Certificates of Analysis (COA) detailing parameters such as protein content, enzymatic activity (U/mg), and purity are provided to guarantee compliance with research and development standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent moisture absorption, which can affect activity. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Positive for cytosine deamination
Protein Content (Biuret)	>90%
Specific Activity	≥ X U/mg protein
Purity (SDS-PAGE)	Single major band
pH (in solution)	7.0 - 7.5
Endotoxin (LAL)	< 1.0 EU/μg (for research grades)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.