Description

Ec 4.1.1.18 Type I is a highly purified form of the enzyme Malate Dehydrogenase (MDH). This product is essential for catalyzing the reversible oxidation of malate to oxaloacetate, a critical reaction in the citric acid cycle and gluconeogenesis. It is a vital reagent for research, diagnostic, and industrial biocatalytic applications requiring consistent enzymatic activity and high specificity. Key users include biochemical research laboratories, in-vitro diagnostic (IVD) kit manufacturers, and bioprocessing facilities.

Application

• Clinical Diagnostics: A key component in enzymatic assay kits for the quantitative determination of metabolites like malate, oxaloacetate, or NAD/NADH in serum and plasma samples.
• Biochemical Research: Used as a standard reagent in metabolic pathway studies, enzyme kinetics experiments, and cellular respiration research.
• Food & Beverage Analysis: Employed in enzymatic methods for the detection and quantification of specific organic acids in food quality control and safety testing.
• Pharmaceutical Development: Utilized in enzymatic synthesis and as a tool enzyme in drug discovery platforms targeting metabolic disorders.
• Bioprocessing Monitoring: Integrated into biosensor systems for real-time monitoring of fermentation processes and metabolic flux analysis.

Basic Information

Item	Detail
Product Name	Ec 4.1.1.18 Type I
CAS No.	9024-76-4
Molecular Formula	Protein (Enzyme)
Molecular Weight	~70 kDa (Subunit dependent)
Synonyms	Malate Dehydrogenase; MDH; L-Malate:NAD+ Oxidoreductase; EC 4.1.1.18; Malic Dehydrogenase; Malate NAD Oxidoreductase; Type I Malate Dehydrogenase; Mitochondrial Malate Dehydrogenase; NAD-dependent Malate Dehydrogenase
EINECS	Contact for details

Quality Control

Our Ec 4.1.1.18 Type I is manufactured under strict quality management systems. Each lot undergoes rigorous testing to ensure high specific activity, purity, and stability. Quality parameters are verified against in-house specifications designed for research and diagnostic use. A comprehensive Certificate of Analysis (COA) detailing assay results, purity, and stability data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This material is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation and moisture uptake. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (IR/Activity)	Conforms
Specific Activity	> 800 units/mg protein
Purity (SDS-PAGE)	> 95%
Protein Content (Biuret)	Contact for details
Stabilizers/Additives	May contain buffer salts (e.g., phosphate)
Solubility	Soluble in water or specified buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.