Description

Threonine Deaminase (Crude Enzyme) is a biocatalyst that specifically deaminates L-threonine to form α-ketobutyrate and ammonia. This enzymatic activity is crucial for research and industrial processes involving amino acid metabolism and biosynthesis. It is primarily utilized by biotechnology firms, academic research institutions, and manufacturers in the life science and diagnostic reagent sectors for biochemical studies, pathway analysis, and enzyme cascade development.

Application

• Biochemical Research & Pathway Analysis: Used as a key reagent in studying the biosynthesis and degradation pathways of branched-chain amino acids.
• Enzyme Kinetics Studies: Employed to investigate reaction mechanisms, inhibition, and catalytic efficiency of deaminase enzymes.
• Diagnostic Kit Manufacturing: Serves as a component in enzymatic assay kits for clinical diagnostics and metabolic disorder testing.
• Biosynthesis & Biocatalysis: Applied in microbial fermentation and synthetic biology platforms for the production of specialty chemicals and amino acid derivatives.
• Educational & Teaching Laboratories: Provides a model enzyme for demonstrating enzymatic principles in university and college laboratory courses.
• Food & Feed Science Research: Investigated for potential applications in modifying amino acid profiles or reducing anti-nutritional factors.

Basic Information

Product Name	Threonine Deaminase (Crude Enzyme)
CAS No.	9024-34-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	L-Threonine Deaminase; Threonine Dehydratase; L-Threonine Hydro-Lyase (Deaminating); Biodegradative Threonine Deaminase; TDH; EC 4.3.1.19; IlvA (gene product); Serine/Threonine Deaminase; Amino Acid Deaminase
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure batch-to-batch consistency and activity for research and industrial use. Each lot is characterized for specific enzymatic activity and protein content. Certificates of Analysis (COA) detailing activity units, purity, and buffer composition are available upon request. Our quality system is designed to meet the needs of research and bioprocessing applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive). Avoid repeated freeze-thaw cycles. For optimal performance, aliquot the product upon receipt and store under recommended conditions.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Specific Activity	≥ X units/mg protein (Contact for details)
Protein Content	Determined by Bradford or BCA assay
Purity (SDS-PAGE)	Crude enzyme preparation
Buffer Composition	Tris-HCl, Glycerol, DTT (typical)
pH	7.0 - 8.0
Stability	Stable for XX months at -20°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.