Description

Triose-Phosphate Isomerase is a crucial glycolytic enzyme that catalyzes the reversible interconversion of dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate. This reaction is fundamental to cellular energy metabolism, making the enzyme an essential reagent for biochemical research and diagnostic applications. It is primarily utilized by researchers in biochemistry, molecular biology, and enzymology, as well as by manufacturers of diagnostic kits and biocatalysts for specialized synthesis.

Application

• Biochemical Research: A fundamental tool for studying glycolysis, enzyme kinetics, and metabolic pathways in vitro.
• Diagnostic Kit Manufacturing: Used as a key component in clinical diagnostic assays for measuring metabolites like triose phosphates or related enzymes.
• Enzymatic Synthesis: Employed as a biocatalyst in the production of specific sugar phosphates for pharmaceutical or fine chemical synthesis.
• Metabolic Pathway Analysis: Essential for reconstituting and analyzing glycolytic flux in cell-free systems or metabolic engineering studies.
• Educational & Teaching Kits: Supplied for university-level laboratory courses in biochemistry and enzymology.
• Quality Control Reagent: Used as a standard or control reagent in pharmaceutical and biotechnology quality control laboratories.

Basic Information

Product Name	Triose-Phosphate Isomerase
CAS No.	9023-78-3
Molecular Formula	N/A (Protein)
Molecular Weight	~27 kDa (monomer)
Synonyms	Triosephosphate Isomerase; TIM; TPI; D-Glyceraldehyde-3-phosphate ketol-isomerase; Phosphotriose isomerase; Triose phosphate mutase; TIMP; EC 5.3.1.1
EINECS	Contact for details

Quality Control

Our Triose-Phosphate Isomerase is produced and tested under stringent conditions to ensure high activity, purity, and lot-to-lot consistency suitable for research and industrial applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing specific activity, purity level (via SDS-PAGE), and functional performance data. We adhere to relevant quality management standards to guarantee product reliability.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation. Aliquot reconstituted solutions to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or solution
Identification (Activity)	Conforms (Specific activity provided on COA)
Specific Activity	> 5,000 units/mg protein (Typical)
Purity (SDS-PAGE)	> 95%
Protein Content (Biuret)	Provided on COA
Solubility	Soluble in water or specified buffer
Stabilizers/Additives	May contain buffer salts (e.g., phosphate, Tris)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.