Description

Phosphoribosyltransferase, Hypoxanthine is a crucial enzyme in the purine salvage pathway, specifically catalyzing the reversible phosphoribosylation of hypoxanthine to inosine monophosphate (IMP). This enzymatic activity is fundamental for nucleotide metabolism and recycling, making it an essential reagent for biochemical research and diagnostic applications. It is primarily utilized by researchers and manufacturers in the life sciences, pharmaceutical development, and in vitro diagnostic (IVD) kit production.

Application

• Biochemical Research: A key reagent for studying purine metabolism, enzyme kinetics, and nucleotide synthesis pathways in vitro.
• Diagnostic Assay Manufacturing: Used as a critical component in the production of enzymatic assay kits for clinical diagnostics, particularly for measuring substrates or enzymes in the purine pathway.
• Pharmaceutical Development: Employed in screening and research for drug discovery targeting nucleotide metabolism disorders, such as those related to gout or certain cancers.
• Enzymology Studies: Serves as a standard or specific enzyme for teaching and research into transferase mechanisms and cofactor requirements.
• Microbiology & Cell Culture: Used in studies of microbial or cellular systems where purine synthesis is a focus, including genetic and metabolic engineering.

Basic Information

Item	Detail
Product Name	Phosphoribosyltransferase, Hypoxanthine
CAS No.	9016-12-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Hypoxanthine Phosphoribosyltransferase; HPRT; HGPRT; Hypoxanthine-guanine phosphoribosyltransferase; EC 2.4.2.8; IMP pyrophosphorylase; Inosinic pyrophosphorylase; 6-Hydroxypurine phosphoribosyltransferase
EINECS	Contact for details

Quality Control

Our Phosphoribosyltransferase, Hypoxanthine is produced and tested under stringent quality management protocols. Each lot undergoes rigorous analytical testing to ensure high specific activity, purity, and functional stability. Certificates of Analysis (COA) detailing activity units, protein content, and purity are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Functional activity confirmed
Specific Activity	≥ [Value] units/mg protein
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	≥ 90%
Stabilizers/Buffer	Potassium phosphate, DTT, Glycerol (typical)
pH	7.0 - 7.5
Biological Contaminants	Negative for DNase, RNase, Protease

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.