Description

Ec 2.7.7.12 is a high-purity enzyme preparation, specifically a nucleotidyltransferase, identified by the CAS number 9016-11-9. This product is essential for catalyzing key biochemical reactions involving the transfer of nucleotide residues, making it a critical reagent in advanced life science research and bioprocess development. It is primarily utilized by biotechnology, pharmaceutical, and diagnostic industries for applications in molecular biology, nucleic acid synthesis, and enzymatic assay development.

Application

• Molecular Biology Research: Used as a key enzyme in the synthesis and modification of DNA and RNA oligonucleotides.
• Diagnostic Kit Manufacturing: Serves as a core component in enzymatic assays and diagnostic test kits for pathogen detection.
• Pharmaceutical Development: Employed in the research and production of nucleotide-based therapeutics and vaccines.
• Biocatalysis: Applied in the enzymatic synthesis of nucleotide analogs and other high-value biochemical intermediates.
• Enzymology Studies: Used as a standard or reagent for studying enzyme kinetics, mechanisms, and inhibitor screening.
• Food Safety Testing: Incorporated into enzymatic methods for the detection of specific contaminants or analytes.

Basic Information

Product Name	Ec 2.7.7.12
CAS No.	9016-11-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Nucleotidyltransferase; Polyribonucleotide Nucleotidyltransferase; Polynucleotide Phosphorylase; PNPase; EC 2.7.7.8 (Note: Older classification may be referenced); RNA Nucleotidyltransferase; Poly(A) Polymerase (in specific contexts); Terminal Nucleotidyltransferase
EINECS	Contact for details

Quality Control

Our Ec 2.7.7.12 is produced under stringent quality management systems. Each batch undergoes comprehensive analytical testing to ensure high specific activity, purity, and functional consistency. Certificates of Analysis (COA) detailing assay results, purity profiles, and stability data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to prevent condensation. For short-term use, store at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	≥ [Value] units/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Content (Bradford)	Reported per batch
Endotoxin Level (LAL)	< 1.0 EU/μg (for research-grade)
Solubility	Clear, colorless solution in specified buffer
Storage Buffer	Tris-HCl, Glycerol, DTT (typical)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.