Description

Rna Polymerase CAS NO 9014-24-8 is a critical enzyme complex responsible for the transcription of DNA into RNA, a fundamental process in gene expression. This product is essential for researchers and manufacturers requiring high-fidelity, high-activity enzyme preparations for molecular biology applications. It is primarily utilized by the biotechnology, pharmaceutical, and life sciences research industries for applications ranging from basic research to large-scale in vitro transcription processes.

Application

• In Vitro Transcription (IVT): For the synthesis of messenger RNA (mRNA), guide RNA (gRNA), and other RNA molecules in therapeutic and vaccine development pipelines.
• Molecular Biology Research: Essential for studying gene regulation, promoter analysis, and RNA synthesis in academic and industrial R&D laboratories.
• RNA Probe Synthesis: Generation of labeled RNA probes for use in techniques such as Northern blotting, in situ hybridization, and microarray analysis.
• Structural Biology Studies: Used in biochemical and biophysical assays to investigate transcription mechanisms and enzyme kinetics.
• Diagnostic Kit Manufacturing: A key component in the production of kits for pathogen detection and genetic testing that rely on RNA amplification or detection.
• Synthetic Biology: For the construction of genetic circuits and the production of RNA components in engineered biological systems.

Basic Information

Item	Detail
Product Name	Rna Polymerase
CAS No.	9014-24-8
Molecular Formula	N/A (Enzyme Complex)
Molecular Weight	~500 kDa (Varies by source and subunit composition)
Synonyms	RNAP; DNA-Directed RNA Polymerase; Transcriptase; RNA Transcriptase; Ribonucleic Acid Polymerase; DNA-Dependent RNA Polymerase; Nucleotidyltransferase (RNA); EC 2.7.7.6
EINECS	Contact for details

Quality Control

Our Rna Polymerase is produced and tested under stringent quality management systems. Each batch undergoes rigorous analytical testing to ensure high specific activity, purity, and absence of contaminating nucleases (DNase and RNase). Certificates of Analysis (COA) detailing activity units, purity by SDS-PAGE, and functional performance in standard transcription assays are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive); avoid repeated freeze-thaw cycles and equilibrate to room temperature before opening to prevent condensation. Aliquotting upon receipt is recommended for frequent use.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Specific Activity	> 50,000 units/mg protein
Purity (SDS-PAGE)	> 95%
DNase Contamination	Not detectable
RNase Contamination	Not detectable
Buffer Composition	50 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.9 at 25°C
Functional Test	Produces full-length transcript from control template

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.