Description

Pyruvate Dehydrogenase is a critical multi-enzyme complex that catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA, linking glycolysis to the citric acid cycle. This reaction is fundamental to cellular energy metabolism, making the enzyme indispensable for research and industrial processes focused on bioenergetics and metabolic engineering. It is essential for biopharmaceutical manufacturers, diagnostic kit developers, and academic research institutions studying mitochondrial function, metabolic disorders, and enzyme kinetics. The product is supplied as a high-purity, lyophilized powder to ensure optimal activity and stability for demanding applications.

Application

• Biochemical Research & Assay Development: Used as a key reagent in enzymatic assays to study metabolic pathways, enzyme inhibition, and mitochondrial respiration.
• Diagnostic Manufacturing: Incorporated into clinical diagnostic kits for the measurement of pyruvate, lactate, or related metabolites in blood and other biological samples.
• Pharmaceutical R&D: Employed in drug discovery screens targeting metabolic diseases, cancer metabolism, and as a tool for producing specific biochemical intermediates.
• Enzyme Engineering & Biotechnology: Serves as a model system for studying multi-enzyme complexes and for applications in synthetic biology and biocatalysis.
• Educational & Teaching Laboratories: Provided for university-level biochemistry and molecular biology courses to demonstrate central metabolic reactions.
• Food & Nutritional Science Research: Utilized in studies investigating the metabolic effects of nutrients and supplements on cellular energy production.

Basic Information

Product Name	Pyruvate Dehydrogenase
CAS No.	9014-20-4
Molecular Formula	N/A (Protein Complex)
Molecular Weight	~8,000,000 Da (Complex)
Synonyms	Pyruvate Dehydrogenase Complex; PDC; Pyruvate Dehydrogenase (Lipoamide); Pyruvate Dehydrogenase Multienzyme Complex; 2-Oxoacid Dehydrogenase; Pyruvic Dehydrogenase; Pyruvate:lipoamide oxidoreductase (decarboxylating and acceptor-acetylating); EC 1.2.4.1
EINECS	Contact for details

Quality Control

Our Pyruvate Dehydrogenase is manufactured under strict quality management systems. Each lot is rigorously tested to ensure high specific activity, purity, and functional integrity. A comprehensive Certificate of Analysis (COA) is provided, detailing key parameters such as specific activity (U/mg protein), protein concentration, and absence of contaminating enzymatic activities. While not intended as a drug substance, production follows principles aligned with ISO 13485 and cGMP guidelines for reagent-grade enzymes to guarantee batch-to-batch consistency and reliability for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C or below. Upon reconstitution, aliquot and store at -70°C to minimize activity loss. The product is hygroscopic (moisture-sensitive); always allow the vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Activity)	Catalyzes the conversion of pyruvate to acetyl-CoA
Specific Activity	≥ 1.0 U/mg protein (typical range 1.5 - 3.0 U/mg)
Protein Content (Biuret)	≥ 70%
Solubility	Clear to slightly opalescent solution in specified buffer
Contaminating Activities (Lactate Dehydrogenase, NADH Oxidase)	≤ 0.1% of Pyruvate Dehydrogenase activity
pH (in reconstitution buffer)	7.0 - 7.5

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.