Description

Ec 6.4.1.1 CAS NO 9014-19-1 is a high-purity enzyme preparation, specifically Pyruvate Carboxylase. This biocatalyst is essential for critical biochemical pathways, including gluconeogenesis and anaplerotic reactions, making it a vital reagent for research and industrial bioprocesses. It is primarily utilized by biotechnology firms, pharmaceutical R&D laboratories, and academic institutions for metabolic studies, diagnostic kit manufacturing, and the synthesis of fine chemicals.

Application

• Biochemical Research & Metabolic Pathway Analysis: A key reagent for in vitro studies of gluconeogenesis, lipogenesis, and neurotransmitter synthesis.
• Diagnostic Kit Manufacturing: Used as a core enzymatic component in clinical diagnostic assays for measuring metabolites like pyruvate, oxaloacetate, or related disorders.
• Pharmaceutical Development: Employed in the research and production of therapeutics targeting metabolic diseases.
• Enzyme Engineering & Biocatalysis: Serves as a model enzyme or catalyst for developing novel biosynthetic routes in white biotechnology.
• Academic & Educational Use: Standardized enzyme for teaching advanced biochemistry and enzymology principles.
• Food & Nutritional Science Research: Applied in studies related to amino acid metabolism and nutritional biochemistry.

Basic Information

Product Name	Pyruvate Carboxylase
CAS No.	9014-19-1
Molecular Formula	N/A (Protein)
Molecular Weight	~500,000 Da (Tetramer, species-dependent)
Synonyms	Pyruvate Carboxylase; PC; Pyruvate:carbon dioxide ligase (ADP-forming); EC 6.4.1.1; Pyruvic carboxylase; Pyruvate decarboxylase (not recommended); Biotin-containing subunit of pyruvate carboxylase
EINECS	Contact for details

Quality Control

Our Pyruvate Carboxylase is produced and tested under stringent quality management systems. Each lot is characterized for specific activity, purity, and stability to ensure performance consistency for critical applications. A comprehensive Certificate of Analysis (COA) detailing specific activity (U/mg), protein content, purity (by SDS-PAGE), and biotin content is provided with every shipment to guarantee traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation. Avoid repeated freeze-thaw cycles. For specific storage buffers or conditions, please refer to the accompanying COA.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	≥ 5-20 U/mg protein (Lot-specific)
Protein Content (Biuret)	≥ 90%
Purity (SDS-PAGE)	Single major band
Stabilizers	BSA or Glycerol present
Biotin Content	~1 mol per subunit
pH (in solution)	6.5 - 7.5

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.