Description

Ec 3.5.1.11 CAS NO 9014-06-6 is a high-purity enzyme preparation, specifically a Penicillin Amidase (also known as Penicillin Acylase), which is critical for the biocatalytic synthesis and modification of β-lactam antibiotics. This enzyme enables the highly selective hydrolysis and synthesis of key pharmaceutical intermediates, offering a sustainable and efficient alternative to traditional chemical processes. It is an essential biocatalyst for manufacturers in the pharmaceutical, fine chemical, and research sectors seeking to develop or produce semi-synthetic penicillins and cephalosporins with high stereoselectivity and yield.

Application

• Semi-Synthetic Antibiotic Production: Key biocatalyst for the enzymatic hydrolysis of Penicillin G or V to yield 6-aminopenicillanic acid (6-APA), the crucial core intermediate for amoxicillin, ampicillin, and other derivatives.
• Cephalosporin Synthesis: Used in the enzymatic acylation processes for the production of advanced cephalosporin antibiotics, enhancing purity and reducing by-products.
• Fine Chemical Synthesis: Employed in the resolution of racemic mixtures and the synthesis of chiral intermediates for active pharmaceutical ingredients (APIs) beyond β-lactams.
• Biocatalytic Process Development: Serves as a model enzyme in R&D for designing and optimizing immobilized enzyme reactors and continuous flow biocatalysis systems.
• Diagnostic Reagents: Utilized in the manufacture of enzymatic assay kits for the detection and quantification of penicillin residues.
• Enzyme Immobilization Studies: A preferred enzyme for research on carrier materials and techniques for enzyme stabilization and reuse in industrial processes.

Basic Information

Product Name	Ec 3.5.1.11 CAS NO 9014-06-6
CAS No.	9014-06-6
Molecular Formula	N/A (Enzyme)
Molecular Weight	~80,000 Da (Approximate, varies by source and form)
Synonyms	Penicillin Amidase; Penicillin Acylase; Penicillin Amidohydrolase; Benzylpenicillin Acylase; Penicillin G Acylase; PGA; EC 3.5.1.11; Penicillin V Acylase; α-Acylamino-β-lactam Acylhydrolase
EINECS	Contact for details

Quality Control

Our Penicillin Amidase is produced and tested under strict quality management systems to ensure batch-to-batch consistency and performance. Each lot is characterized for critical parameters including specific activity, protein content, and purity profile. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specifications for activity (U/mg), pH optimum, temperature stability, and absence of specified microbial contaminants. We support compliance with cGMP and ISO 9001 standards for pharmaceutical ingredient manufacturers.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at 2-8°C. This product is hygroscopic (moisture-sensitive). Avoid repeated freeze-thaw cycles for liquid formulations. Keep container sealed under inert atmosphere if specified for certain purified forms.

Specification

Item	Specification
Appearance	Lyophilized powder or clear solution
Identification (IR)	Conforms to standard
Specific Activity	≥ 50 U/mg protein (Typical)
Protein Content (Biuret)	≥ 90%
pH Optimum	7.5 - 8.5
Temperature Optimum	37 - 45 °C
Loss on Drying	≤ 5.0 %
Total Microbial Count	≤ 1000 CFU/g

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.