Description

Ec 3.4.21.14 is a highly purified proteolytic enzyme preparation, also known as trypsin. This product is essential for numerous biochemical and biotechnological processes due to its specific cleavage of peptide bonds, making it a critical reagent for protein digestion, cell culture, and tissue dissociation. It is widely utilized by research institutions, pharmaceutical manufacturers, and diagnostic kit producers who require consistent enzymatic activity and high purity for reproducible results. The compound is supplied with a CAS number of 9014-01-1.

Application

• Cell Culture & Tissue Dissociation: Primary application for detaching adherent mammalian cells from culture vessels without damaging cell viability.
• Protein Biochemistry & Proteomics: Used for protein digestion and peptide mapping in mass spectrometry-based protein identification and analysis.
• Pharmaceutical Manufacturing: Employed in downstream processing for the refinement of biological products, including insulin and vaccine production.
• Diagnostic Reagents: A key component in clinical diagnostic kits for various assays requiring specific proteolytic activity.
• Food Industry Processing: Applied in food technology for tenderization and the modification of protein-based food products.
• Research & Development: Indispensable tool in life science research for studying protein structure, function, and cellular mechanisms.

Basic Information

Item	Detail
Product Name	Ec 3.4.21.14
CAS No.	9014-01-1
Molecular Formula	N/A (Enzyme Preparation)
Molecular Weight	~23,300 Da (for bovine trypsin)
Synonyms	Trypsin; Trypsin from Bovine Pancreas; Parenzyme; Tryptar; Trypure; Protease, Serine; EC 3.4.21.4; Tryptase
EINECS	232-650-8

Quality Control

Our Ec 3.4.21.14 is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive testing for enzymatic activity, purity, and the absence of contaminating proteases. Certificates of Analysis (COA) detailing specifications such as specific activity, protein content, and impurity profiles are available upon request. The product meets relevant standards for research and industrial use.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This material is hygroscopic (moisture-sensitive) and should be kept under dry conditions to maintain stability and activity. For long-term storage, desiccants are recommended.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms
Specific Activity	> 10,000 BAEE units/mg protein
Protein Content (Biuret)	> 90%
Loss on Drying	< 5.0%
Chymotrypsin Activity	< 1.0%
Solubility	Clear, colorless solution in 1mM HCl

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.