Description

Hemocyanin, Keyhole Limpet is a high-molecular-weight, copper-containing respiratory protein extracted from the marine gastropod *Megathura crenulata*. This unique biochemical is a critical reagent in immunological research and diagnostics, valued for its potent immunogenicity and utility as a carrier protein. It is primarily utilized by research institutions, diagnostic manufacturers, and biopharmaceutical companies for applications in antibody production, vaccine development, and immunological assays.

Application

• Immunological Research: Widely employed as a carrier protein to enhance the immunogenicity of haptens in polyclonal and monoclonal antibody production.
• Vaccine Adjuvant Development: Investigated for its immune-stimulating properties in preclinical vaccine research and immunotherapeutic studies.
• Diagnostic Assays: Used as a coating antigen or a blocking agent in enzyme-linked immunosorbent assays (ELISAs) and other immunoassays.
• Immunohistochemistry (IHC): Serves as a valuable tool for studying immune responses and cell surface markers in tissue samples.
• T-Cell Stimulation Studies: Acts as a model antigen in immunological research to study T-cell activation and proliferation.
• Bioconjugation: A robust platform for conjugating peptides, carbohydrates, and other small molecules for immunological applications.

Basic Information

Product Name	Hemocyanin, Keyhole Limpet
CAS No.	9013-72-3
Molecular Formula	Contact for details
Molecular Weight	> 3,000 kDa (Multi-subunit complex)
Synonyms	KLH; Keyhole Limpet Hemocyanin; *Megathura crenulata* Hemocyanin; Hemocyanin from Keyhole Limpet; Limulus Hemocyanin (common misnomer); Hc; Immunogenic Carrier Protein; Respiratory Protein KLH
EINECS	Contact for details

Quality Control

Our Hemocyanin, Keyhole Limpet is produced and tested under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot is characterized for key parameters including protein concentration, purity, and immunological reactivity. Certificates of Analysis (COA) detailing specifications and test results are provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at 2-8°C. Reconstituted solutions should be aliquoted and stored at -20°C or below to prevent degradation. The product is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after each use.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR/Spectroscopy)	Conforms to standard for Hemocyanin
Protein Content (Biuret/BCA)	> 95%
Purity (SDS-PAGE/Gel Filtration)	Major band corresponding to KLH subunits
Endotoxin Level (LAL)	< 10 EU/mg
Solubility	Soluble in saline or buffer solutions
Biological Activity (Immunogenicity)	Tested in standard immunization model

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.