Description

Streptavidin CAS NO 9013-20-1 is a highly purified, tetrameric protein derived from *Streptomyces avidinii* with an exceptionally high affinity for biotin. This strong and specific interaction is the cornerstone of numerous detection, purification, and diagnostic systems in life science research and biotechnology. It is an indispensable reagent for professionals in molecular biology, immunoassay development, and targeted drug delivery systems.

Application

• Molecular Diagnostics & Immunoassays: Used as a universal detection conjugate in ELISA, lateral flow assays, and Western blotting for signal amplification.
• Affinity Purification & Pull-Down Assays: Immobilized on solid supports (beads, plates) to isolate biotinylated proteins, nucleic acids, or other molecules.
• Cell Biology & Imaging: Conjugated to fluorophores, enzymes, or nanoparticles for highly specific labeling and visualization of cell surface receptors or intracellular targets.
• Biosensor Development: Serves as a robust linker layer for immobilizing biotinylated capture probes on sensor surfaces.
• Drug Delivery & Targeting: Utilized in pretargeting strategies where streptavidin-conjugated therapeutics bind to biotinylated targets in vivo.
• Next-Generation Sequencing (NGS): Key component in sample preparation workflows for capturing biotin-labeled DNA or RNA fragments.
• Flow Cytometry: Employed in multi-parameter staining protocols via biotin-streptavidin binding to increase detection sensitivity.
• Microarray Technology: Used to attach biotinylated samples or probes to array surfaces for high-throughput analysis.

Basic Information

Product Name	Streptavidin
CAS No.	9013-20-1
Molecular Formula	N/A (Protein)
Molecular Weight	~52,800 g/mol (tetramer)
Synonyms	Streptavidin (from *Streptomyces avidinii*); Avidin-like protein from *Streptomyces*; Streptavidin tetramer; Streptavidin, core; SA; STA; Streptavidin, recombinant
EINECS	Contact for details

Quality Control

Our Streptavidin is manufactured under strict quality systems to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous testing, including purity assessment by SDS-PAGE and HPLC, functional activity measurement via biotin-binding capacity, and endotoxin level testing. A comprehensive Certificate of Analysis (COA) detailing purity, activity, and specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at 2-8°C. Reconstituted solutions should be aliquoted and stored at -20°C or below to prevent repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive).

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Purity (SDS-PAGE)	≥ 95%
Assay (Biotin-Binding Capacity)	≥ 13 units/mg protein
Protein Content (Biuret)	Contact for details
Solubility	Soluble in water or neutral buffer
Endotoxin	< 1.0 EU/mg
Identification (IR / Electrophoresis)	Conforms

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.