Description

Phosphoenolpyruvatecarboxylaseex CAS NO 9013-08-5 is a high-purity enzyme preparation, specifically phosphoenolpyruvate carboxylase, which plays a critical catalytic role in the carboxylation of phosphoenolpyruvate. This enzyme is essential for biochemical research, diagnostic reagent formulation, and enzymatic synthesis processes. It is primarily required by biotechnology companies, pharmaceutical R&D laboratories, and manufacturers of in-vitro diagnostic (IVD) kits who demand reliable and consistent enzymatic activity for their critical applications.

Application

• Biochemical Research & Assay Development: Used as a key reagent in enzymatic studies, metabolic pathway analysis, and the development of coupled enzyme assays.
• Diagnostic Reagent Manufacturing: A critical component in the formulation of clinical chemistry reagents for measuring specific metabolites in blood and other biological samples.
• Pharmaceutical Synthesis: Employed in biocatalytic processes for the synthesis of chiral intermediates and active pharmaceutical ingredients (APIs).
• Food & Beverage Analysis: Utilized in enzymatic methods for the quantitative determination of organic acids and other compounds in food quality control.
• Academic & Teaching Laboratories: Serves as a standard enzyme for educational purposes in biochemistry and molecular biology courses.
• Agricultural Biotechnology: Applied in research focused on plant metabolism and the development of crops with enhanced carbon fixation efficiency.

Basic Information

Product Name	Phosphoenolpyruvatecarboxylaseex
CAS No.	9013-08-5
Molecular Formula	N/A (Enzyme)
Molecular Weight	Varies by source and preparation
Synonyms	Phosphoenolpyruvate carboxylase; PEPCase; PEPC; PEP carboxylase; Phosphoenolpyruvic carboxylase; Orthophosphate:oxaloacetate carboxy-lyase (phosphorylating); EC 4.1.1.31; PEPC enzyme
EINECS	Contact for details

Quality Control

Our Phosphoenolpyruvatecarboxylaseex is produced and tested under stringent quality management systems. Each batch is characterized for specific activity, purity, and stability to ensure it meets the rigorous demands of research and industrial applications. Certificates of Analysis (COA) detailing activity units/mg protein, purity by SDS-PAGE, and absence of contaminating activities are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent moisture absorption, which can lead to loss of activity. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or suspension
Specific Activity	≥ [Value] units/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Content (Biuret)	Reported on COA
Stabilizers / Buffer	Potassium phosphate, Glycerol, DTT (as applicable)
Contaminating Activities	Absence of significant PEP phosphatase, malate dehydrogenase, etc.

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.