Description

Nitrate Reductase CAS NO 9013-03-0 is a critical oxidoreductase enzyme that catalyzes the reduction of nitrate (NO₃⁻) to nitrite (NO₂⁻), a fundamental step in the nitrogen cycle. This enzymatic activity is essential for research and industrial processes involving nitrogen metabolism and analysis. It is a vital reagent for laboratories and manufacturers in the environmental monitoring, agricultural science, and biochemical research sectors.

Application

• Environmental Water Testing: Key component in enzymatic assay kits for the quantitative determination of nitrate levels in water, soil, and wastewater to monitor pollution and eutrophication.
• Agricultural Research: Used in studies of plant nitrogen assimilation, fertilizer efficiency, and the metabolic pathways of nitrate in crops and soil microorganisms.
• Clinical & Food Analysis: Employed in diagnostic methods for detecting nitrate/nitrite in biological fluids (e.g., saliva, urine) and in food safety testing for preserved meats and vegetables.
• Biochemical Research: Serves as a model enzyme for studying molybdenum cofactor-containing enzymes, electron transfer mechanisms, and anaerobic respiration pathways.
• Industrial Biocatalysis: Investigated for potential use in biosensor development and bioelectrochemical systems for nitrate removal or conversion.

Basic Information

Product Name	Nitrate Reductase
CAS No.	9013-03-0
Molecular Formula	Protein (Enzyme)
Molecular Weight	Varies by source (typically ~100-200 kDa for subunits)
Synonyms	NADH-Nitrate Reductase; Nitrate Reductase (NADH); Assimilatory Nitrate Reductase; EC 1.7.1.1; Nitrate Reductase NADH-Dependent; Ferredoxin-Nitrate Reductase; NR
EINECS	Contact for details

Quality Control

Our Nitrate Reductase is produced and tested under stringent conditions to ensure high specific activity and batch-to-batch consistency. Each lot is characterized for key enzymatic parameters. A comprehensive Certificate of Analysis (COA) detailing purity, activity (units/mg protein), and stability is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after use to prevent moisture absorption, which can affect activity.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Catalyzes reduction of nitrate to nitrite
Specific Activity	≥ [Value] units/mg protein
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	Single major band
Stabilizers / Buffer	May contain glycerol, buffer salts
Storage Buffer	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.