Description

Phosphorylase B is a purified enzyme derived from rabbit muscle, critical for catalyzing the reversible phosphorolysis of glycogen to glucose-1-phosphate. This product is essential for fundamental biochemical research and diagnostic applications, providing a reliable standard for studying glycogen metabolism and enzyme kinetics. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic kit manufacturers requiring high-purity enzymatic reagents.

Application

• Biochemical Research Standard: Used as a reference standard in enzymatic assays to study glycogen breakdown and synthesis pathways.
• Diagnostic Reagent Manufacturing: A key component in the production of clinical diagnostic kits for assessing glycogen storage diseases and metabolic disorders.
• Pharmaceutical Development: Employed in drug discovery research targeting metabolic enzymes and for screening potential modulators of phosphorylase activity.
• Enzyme Kinetics Studies: Essential for investigating reaction mechanisms, inhibitor effects, and allosteric regulation of phosphorylase enzymes.
• Educational & Teaching Laboratories: Provides a model enzyme for university-level biochemistry courses demonstrating enzyme purification and characterization techniques.
• Biotechnology: Used in enzymatic synthesis and biocatalysis processes for producing specific sugar phosphates.

Basic Information

Product Name	Phosphorylase B
CAS No.	9012-69-5
Molecular Formula	N/A (Protein)
Molecular Weight	~194,000 Da (dimer)
Synonyms	Glycogen Phosphorylase B; 1,4-α-D-Glucan:Orthophosphate α-D-Glucosyltransferase B; Phosphorylase B from Rabbit Muscle; α-Glucan Phosphorylase B; Glycogen Phosphorylase Isozyme B; GPBB (Glycogen Phosphorylase Brain Type); Phosphorylase (rabbit muscle); EC 2.4.1.1
EINECS	Contact for details

Quality Control

Our Phosphorylase B is produced and tested under stringent conditions to ensure high enzymatic activity and purity suitable for research and diagnostic applications. Each lot is characterized for specific activity, protein content, and absence of contaminating enzymatic activities. A comprehensive Certificate of Analysis (COA) detailing assay results, purity by SDS-PAGE, and storage buffer information is provided with every shipment to guarantee traceability and consistency.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C in small aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive). The storage buffer typically contains stabilizing agents such as glycerol. Always handle on ice or in a cold room when thawed.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Identification (Activity)	Catalyzes the phosphorolysis of glycogen
Specific Activity	≥ 25 units/mg protein
Purity (SDS-PAGE)	≥ 90% (Single band at ~97 kDa under reducing conditions)
Protein Concentration	10 - 30 mg/mL (by UV absorbance)
Storage Buffer	50 mM Glycerol 2-phosphate, pH 6.8, containing 1 mM EDTA and 30% (v/v) Glycerol
Contaminating Activities	Negligible protease and phosphatase activity

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.