Description

Cholera Toxin CAS NO 9012-63-9 is a potent protein enterotoxin produced by the bacterium *Vibrio cholerae*, consisting of one A subunit and five B subunits. This compound is a critical research tool for studying G-protein signaling pathways, cellular physiology, and the mechanisms of bacterial pathogenesis. It is essential for researchers and developers in the fields of pharmacology, immunology, vaccine development, and cell biology.

Application

• Biomedical Research: Fundamental tool for investigating G-protein coupled receptor (GPCR) signaling, adenylate cyclase activation, and intracellular cAMP levels.
• Vaccine Development: Used as an immunogen, adjuvant, or challenge agent in the research and development of cholera and other mucosal vaccines.
• Cell Biology Studies: Employed to study cell membrane permeability, vesicular transport, and the cytoskeleton via its action on ADP-ribosylation factors.
• Neuroscience Research: Utilized as a neuronal tracer due to its specific binding to GM1 gangliosides on cell surfaces.
• Immunology Assays: Serves as a potent mucosal immunogen and adjuvant in studies of mucosal immune responses and tolerance.
• Diagnostic Reagent Production: A key component in the development of immunoassays and diagnostic kits for cholera toxin detection.
• Pharmacology & Toxicology: Used to model secretory diarrhea and study the effects of enterotoxins on intestinal epithelial function.
• Drug Discovery Screening: Applied in high-throughput screening assays to identify potential inhibitors of toxin binding or enzymatic activity.

Basic Information

Product Name	Cholera Toxin
CAS No.	9012-63-9
Molecular Formula	Contact for details
Molecular Weight	Approx. 84 kDa (holotoxin)
Synonyms	Cholera Enterotoxin; Vibrio Cholerae Enterotoxin; Choleragen; CTX; CT; Cholera Toxin Subunit B; CTB; Cholera Toxin A Subunit; CTA; Choleragenoid; GM1 Ganglioside-binding Protein
EINECS	Contact for details

Quality Control

Our Cholera Toxin is produced and tested under stringent conditions to ensure high biological activity, purity, and consistency. Each lot is characterized for protein content, subunit composition, and enzymatic activity. A comprehensive Certificate of Analysis (COA) is provided, detailing results for identity, purity (by SDS-PAGE and HPLC), endotoxin levels, sterility (where applicable), and specific biological activity (ADP-ribosyltransferase or GM1-binding ELISA). We adhere to rigorous quality management principles suitable for research and development applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Reconstituted solutions should be aliquoted and stored at ≤ -70°C to avoid repeated freeze-thaw cycles. This product is easily oxidized; handle under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (SDS-PAGE)	Corresponds to standard (A & B subunits)
Purity (HPLC)	≥ 95%
Protein Content (BCA)	≥ 90%
Endotoxin (LAL)	< 1.0 EU/µg
Biological Activity (GM1-ELISA)	Conforms to reference standard
Solubility	Soluble in aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.