Description

Ec 3.5.1.4 CAS NO 9012-56-0 is a high-purity enzyme preparation, specifically an amidase, identified by the Enzyme Commission number 3.5.1.4. This biocatalyst is essential for the selective hydrolysis of amide bonds, enabling precise and efficient synthesis in advanced chemical and pharmaceutical processes. It is a critical reagent for research institutions, fine chemical manufacturers, and pharmaceutical companies developing novel compounds, active pharmaceutical ingredients (APIs), and chiral intermediates.

Application

• Pharmaceutical Synthesis: Key biocatalyst for the enantioselective hydrolysis of amide bonds in the production of chiral amines and amino acids, crucial for active pharmaceutical ingredients (APIs).
• Fine Chemical Manufacturing: Used in the synthesis of specialty chemicals, agrochemical intermediates, and flavor & fragrance compounds requiring high regio- and stereoselectivity.
• Biocatalysis Research & Development: An essential tool for enzymologists and synthetic chemists exploring new enzymatic pathways, reaction optimization, and enzyme engineering.
• Diagnostic Reagent Production: Employed in the formulation of enzymatic assay kits and diagnostic reagents for clinical and research laboratories.
• Biodegradation Studies: Used in environmental science research to study the microbial degradation pathways of amide-containing pollutants.

Basic Information

Product Name	Ec 3.5.1.4
CAS No.	9012-56-0
Molecular Formula	N/A (Enzyme Preparation)
Molecular Weight	Varies (Protein)
Synonyms	Amidase; Acylamide amidohydrolase; EC 3.5.1.4; Aryl acylamidase; Fatty acylamide amidohydrolase; Penicillin amidase (related activity); Acylase (related activity)
EINECS	Contact for details

Quality Control

Our enzyme preparations are produced and tested under stringent quality management systems. Each batch of Ec 3.5.1.4 is characterized for specific activity, protein content, and purity using advanced analytical techniques including SDS-PAGE and HPLC. We ensure consistency and performance for your critical applications. Certificates of Analysis (COA) detailing batch-specific data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C for long-term stability. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent moisture absorption and loss of activity. For extended storage, consider aliquoting and storing at -20°C.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	> 10 U/mg protein (Typical)
Protein Content (Biuret)	> 90%
Purity (SDS-PAGE)	Single major band
pH (in solution)	7.0 - 8.0
Solubility	Soluble in buffer or water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.