Description

Ec 2.7.7.4 CAS NO 9012-39-9 is a high-purity enzyme preparation, specifically a nucleotidyltransferase, essential for biochemical synthesis and research. This product is critical for ensuring precise and reproducible results in enzymatic assays and bioprocessing applications. It is primarily required by biotechnology, pharmaceutical R&D, and diagnostic kit manufacturing industries for applications in molecular biology and nucleic acid synthesis.

Application

• Molecular Biology Research: Used as a key reagent in enzymatic synthesis of oligonucleotides and nucleic acid labeling.
• Diagnostic Kit Manufacturing: Serves as a critical component in the production of kits for genetic testing and pathogen detection.
• Pharmaceutical Development: Employed in the research and development of novel therapeutic agents, including antisense oligonucleotides.
• Biocatalysis: Applied in specialized biocatalytic processes for the synthesis of nucleotide analogs and other high-value biochemicals.
• Academic Research: Utilized in university and institutional labs for studying enzyme kinetics, mechanism, and structure-function relationships.
• Quality Control Testing: Acts as a reference standard in QC laboratories for validating other enzymatic processes and reagents.

Basic Information

Product Name	Ec 2.7.7.4
CAS No.	9012-39-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Polynucleotide Phosphorylase; PNPase; Polyribonucleotide Nucleotidyltransferase; Nucleoside Diphosphate: Polynucleotide Nucleotidyltransferase; EC 2.7.7.8 (obsolete); RNA Nucleotidyltransferase (RNA-directed); Poly(A) Polymerase (in some contexts); Bacterial PNPase
EINECS	Contact for details

Quality Control

Our Ec 2.7.7.4 is produced under stringent quality management systems. Each batch undergoes rigorous analytical testing to ensure high specific activity, purity, and consistency. Certificates of Analysis (COA) detailing assay results, purity profiles, and activity units are available upon request to support your regulatory and quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after each use to prevent moisture uptake. For short-term use, aliquots may be stored at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	≥ [Value] units/mg protein
Protein Content (Biuret)	≥ [Value] %
Purity (SDS-PAGE)	≥ [Value] %
Endotoxin (LAL)	< [Value] EU/mg
Solubility	Clear, colorless solution in specified buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.