Description

β-n-Acetylglucosaminidase is a hydrolytic enzyme that specifically cleaves N-acetyl-β-D-glucosaminide residues from the non-reducing end of glycoproteins, glycolipids, and oligosaccharides. This specificity makes it an indispensable tool for structural analysis and modification of complex carbohydrates in research and industrial biocatalysis. It is essential for laboratories and manufacturers in the biotechnology, pharmaceutical, and diagnostic sectors requiring precise glycan analysis or enzymatic synthesis.

Application

• Glycobiology Research: Used for the sequential deglycosylation and structural elucidation of N-linked and O-linked glycans.
• Biopharmaceutical Development: Employed in the characterization and quality control of therapeutic glycoproteins, including monoclonal antibodies.
• Diagnostic Reagent Manufacturing: A key component in enzymatic assay kits for the detection of specific lysosomal storage disorders.
• Enzymatic Synthesis: Applied in the chemo-enzymatic synthesis of complex oligosaccharides and glycoconjugates.
• Food & Beverage Analysis: Used to analyze and modify polysaccharides and glycoproteins in food products.
• Microbial & Plant Biochemistry: Aids in studying chitin metabolism and cell wall structure in fungi, insects, and plants.

Basic Information

Product Name	β-n-Acetylglucosaminidase
CAS No.	9012-33-3
Molecular Formula	N/A (Enzyme)
Molecular Weight	Varies by source and preparation
Synonyms	N-Acetyl-β-glucosaminidase; β-Hexosaminidase; NAGase; β-NAG; β-N-Acetylhexosaminidase; Chitobiase; 2-Acetamido-2-deoxy-β-D-glucoside acetamidodeoxyglucohydrolase
EINECS	Contact for details

Quality Control

Our β-n-Acetylglucosaminidase is produced and tested under a rigorous quality management system. Each lot is characterized for specific activity, purity, and stability to ensure consistent performance for critical applications. A comprehensive Certificate of Analysis (COA) detailing assay results, purity profiles, and functional activity is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This material is hygroscopic (moisture-sensitive). For long-term stability, consider storage at -20°C. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Positive enzymatic activity
Specific Activity	> X units/mg protein (Lot-specific)
Purity (SDS-PAGE)	> 90%
Protein Content (Bradford)	Lot-specific
pH (in solution)	6.0 - 7.5
Endotoxin	< 1.0 EU/μg (for research grades)
Storage Buffer	Specified per grade (e.g., phosphate, citrate)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.