Description

Lysostaphin is a bacteriolytic enzyme specifically targeting the peptidoglycan cell wall of *Staphylococcus* species, including methicillin-resistant *Staphylococcus aureus* (MRSA). Its unique mechanism of action makes it a critical tool for combating antibiotic-resistant bacterial infections and biofilms. This product is essential for researchers in pharmaceutical development, microbiology, and biotechnology, as well as for manufacturers in the biopharmaceutical and diagnostic sectors.

Application

• Pharmaceutical R&D: Investigated as a novel therapeutic agent for the treatment of staphylococcal infections, particularly those caused by MRSA.
• Microbiological Research: Used as a selective agent in laboratory studies to lyse staphylococcal cells for DNA extraction, protoplast generation, or to study cell wall biosynthesis.
• Biofilm Disruption: Applied in research to degrade and prevent *Staphylococcus* biofilms on medical devices and implants.
• Diagnostic Manufacturing: Incorporated into selective culture media or diagnostic kits for the specific identification of *Staphylococcus* species.
• Veterinary Medicine: Explored for use in treating mastitis in dairy animals caused by staphylococcal pathogens.
• Food Safety Testing: Potential use in assays designed to detect *Staphylococcus aureus* in food samples.

Basic Information

Product Name	Lysostaphin
CAS No.	9011-93-2
Molecular Formula	C₁₈₈H₂₉₈N₅₆O₆₅S₈ (Representative)
Molecular Weight	~25 kDa
Synonyms	Glycyl-glycine endopeptidase; Staphylococcus simulans lytic enzyme; Endo-β-N-acetylglucosaminidase; Peptidoglycan hydrolase; Muramidase; Bacteriolytic enzyme from *Staphylococcus simulans*; LYS; Anti-staphylococcal agent
EINECS	Contact for details

Quality Control

Our Lysostaphin is produced and tested under stringent quality management systems. Each batch is characterized for enzymatic activity, purity, and specificity to ensure consistent performance in research and development applications. A comprehensive Certificate of Analysis (COA) detailing activity (U/mg), purity (by HPLC), protein content, and absence of contaminating nucleases/proteases is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C or below. Reconstituted solutions should be aliquoted and stored at -20°C to -80°C, avoiding repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive).

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to reference standard
Protein Content (Biuret)	> 90%
Specific Activity	> 500 U/mg protein
Purity (SDS-PAGE)	> 95%
Endotoxin (LAL)	< 1.0 EU/µg
Solubility	Soluble in water or buffer (e.g., Tris, PBS)
Biological Activity	Lyses *Staphylococcus aureus* cells

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.