Description

Phytohemagglutinin Pha-M is a purified lectin protein derived from the red kidney bean (*Phaseolus vulgaris*). This high-purity mitogen is essential for stimulating lymphocyte proliferation and agglutinating red blood cells in controlled laboratory settings. It is a critical reagent for researchers and manufacturers in immunology, cell biology, and diagnostic development, requiring consistent biological activity and purity for reproducible results.

Application

• Immunological Research: A standard mitogen used to stimulate T-lymphocyte proliferation in studies of immune cell function, signal transduction, and activation pathways.
• Cell Culture & Cytogenetics: Employed to induce mitosis in peripheral blood lymphocytes for chromosome analysis (karyotyping) and genetic studies.
• Diagnostic Reagent Manufacturing: Used as a key component in hemagglutination assays and other in-vitro diagnostic kits for blood typing and immune response evaluation.
• Biomarker Studies: Utilized to investigate cell surface glycoproteins and carbohydrates due to its specific binding affinity for complex sugars.
• Teaching & Standardization: Serves as a reference standard in academic and quality control laboratories for lymphocyte transformation tests.

Basic Information

Product Name	Phytohemagglutinin Pha-M
CAS No.	9008-97-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	PHA-M; Phytohemagglutinin M; Kidney Bean Lectin; Phaseolus vulgaris Lectin; PHA (from Phaseolus vulgaris); Hemagglutinin; Leukagglutinin; T-cell Mitogen
EINECS	Contact for details

Quality Control

Our Phytohemagglutinin Pha-M is produced under stringent quality management systems to ensure batch-to-batch consistency and high biological activity. Each lot is subjected to rigorous purity and potency testing, including protein assay, hemagglutination activity, and mitogenic activity assays. A comprehensive Certificate of Analysis (COA) detailing purity, biological activity, and endotoxin levels is provided with every shipment to support your research and manufacturing compliance.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. The product is hygroscopic (moisture-sensitive); ensure the container is kept sealed in a dry environment to maintain stability and activity.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to standard
Protein Content (Biuret)	≥ 95%
Hemagglutination Activity	Defined titer per mg protein
Mitogenic Activity	Defined stimulation index
Endotoxin (LAL)	< 10 EU/mg
Solubility	Soluble in water or saline

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.