Description

Methemoglobin is a stable, oxidized form of hemoglobin where the iron is in the ferric (Fe³⁺) state, making it a critical biochemical standard and reagent. This compound is essential for research and diagnostic applications requiring a controlled model of dysfunctional hemoglobin. It is primarily utilized by biochemical research laboratories, diagnostic kit manufacturers, and academic institutions focused on hematology, toxicology, and oxygen transport studies.

Application

• Biochemical Research Standard: Used as a primary standard in spectroscopic studies of hemoglobin derivatives and oxygen-binding kinetics.
• Diagnostic Reagent: Employed in the calibration and development of clinical assays for measuring methemoglobin levels in blood, critical for diagnosing methemoglobinemia.
• Toxicology Studies: Serves as a key substrate for investigating the effects of oxidizing drugs, chemicals, and environmental toxins on hemoglobin.
• Enzyme Activity Assays: Used as a substrate for enzymes like methemoglobin reductase in studies of cellular antioxidant mechanisms.
• Educational Tool: Provides a hands-on reference material for university-level courses in biochemistry, physiology, and medical technology.
• Quality Control Material: Acts as a control substance in the manufacturing and validation of hemoglobin analyzers and point-of-care testing devices.

Basic Information

Product Name	Methemoglobin
CAS No.	9008-37-1
Molecular Formula	C2952H4664N812O832S8Fe4 (Representative for human hemoglobin tetramer)
Molecular Weight	~64,500 Da (for α2β2 tetramer)
Synonyms	Ferrihemoglobin; Hemiglobin; Oxidized Hemoglobin; MetHb; Hi; Hemoglobin (ferric); Erythrocruorin (ferric form)
EINECS	Contact for details

Quality Control

Our Methemoglobin is produced and handled under strict quality protocols to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot is characterized to confirm its oxidized state and purity. A comprehensive Certificate of Analysis (COA) detailing spectroscopic purity, assay, and physical properties is provided with every shipment to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term stability, consider storing desiccated or under an inert atmosphere. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Brown to dark brown lyophilized powder or solid
Identification (UV-Vis)	Spectrum characteristic of methemoglobin
Purity (Spectroscopic)	≥ 90% (as Methemoglobin)
Assay (Hemoglobin Content)	Contact for details
Solubility	Soluble in water or buffer, forming a clear brown solution
Residual Moisture (KF)	< 5.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.