Description

α-Chymotrypsin CAS NO 9004-07-3 is a proteolytic enzyme derived from bovine pancreas, belonging to the serine endopeptidase family. It is valued for its high specificity in cleaving peptide bonds at the carboxyl side of aromatic amino acids like tyrosine, tryptophan, and phenylalanine. This enzyme is essential for research and industrial applications in the pharmaceutical, biotechnology, and diagnostic sectors, where precise protein digestion and analysis are required.

Application

• Pharmaceutical Manufacturing: Used in the production of anti-inflammatory drugs and as a debriding agent in topical ointments for wound cleaning.
• Biotechnological Research: A critical tool for protein sequencing, peptide mapping, and structural analysis in proteomics laboratories.
• Diagnostic Reagent Formulation: Employed in the preparation of specific diagnostic kits for clinical analysis.
• Cell Culture & Tissue Dissociation: Facilitates the gentle dissociation of adherent cells in primary cell culture protocols.
• Food Processing (Enzymatic Modification): Applied in limited, specialized processes for protein hydrolysis to modify functional properties.

Basic Information

Product Name	α-Chymotrypsin
CAS No.	9004-07-3
Molecular Formula	N/A (Protein)
Molecular Weight	Approx. 25,000 Da
Synonyms	α-Chymotrypsin; Chymotrypsin A; Chymotrypsinogen A (activated); α-Chymar; Chymotrypsin from bovine pancreas; EC 3.4.21.1; Quimotripsina; Chymotrypsinum
EINECS	232-671-2

Quality Control

Our α-Chymotrypsin is produced and tested to meet high-purity standards suitable for research and industrial use. Each batch undergoes rigorous analysis including activity assays, purity profiling, and microbiological testing. A Certificate of Analysis (COA) detailing specific parameters such as proteolytic activity (USP units/mg), protein content, and impurity limits is provided to ensure traceability and compliance with your specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. The product is hygroscopic (moisture-sensitive). For long-term stability, consider storage at -20°C in a desiccated environment.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms
Proteolytic Activity	≥ 40 USP units/mg protein
Protein Content (Biuret)	≥ 90%
Loss on Drying	≤ 5.0%
Solubility	Clear, slightly opalescent solution in 1% HCl
Microbial Limits	Meets standard bioburden requirements

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.