Description

Thrombin is a serine protease enzyme that plays a critical role in the blood coagulation cascade by converting soluble fibrinogen into insoluble fibrin strands. This high-purity enzyme is essential for research, diagnostic, and therapeutic applications where precise control of clotting is required. It is a vital reagent for biopharmaceutical manufacturers, clinical laboratories, and life science researchers working in hemostasis, thrombosis, and related fields.

Application

• Hemostatic Agent Formulation: A key active ingredient in topical hemostats, sealants, and glues used in surgical procedures to control bleeding.
• In Vitro Diagnostic (IVD) Kits: Used as a critical component in coagulation analyzers and test kits to measure clotting times and assess hemostatic function.
• Biomedical Research: Essential for studying blood coagulation pathways, platelet aggregation, and the development of antithrombotic therapies.
• Cell Culture & Tissue Engineering: Employed to create fibrin matrices and scaffolds for 3D cell culture and tissue regeneration studies.
• Protein Biochemistry: Used as a specific protease for protein cleavage and analysis in downstream processing.
• Food Science: Applied in certain processes for the recovery of proteins or as a processing aid, adhering to relevant food-grade specifications where applicable.

Basic Information

Product Name	Thrombin
CAS No.	9002-04-4
Molecular Formula	N/A (Protein)
Molecular Weight	~33,700 Da (Single Chain Form)
Synonyms	Thrombin, Bovine; Fibrinogenase; Activated Blood-Coagulation Factor II; Coagulation Factor IIa; E.C. 3.4.21.5; Thrombofort; Topical Thrombin
EINECS	232-650-8

Quality Control

Our Thrombin is manufactured and tested under a strict quality management system. Each batch is rigorously analyzed to ensure it meets high standards for activity, purity, and consistency. Certificates of Analysis (COA) detailing specific activity, purity by HPLC, and absence of specific impurities are provided. Production can be aligned with relevant guidelines for research, diagnostic, or cGMP applications upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder or lyophilized solid
Identification (IR)	Conforms
Specific Activity	> 1000 NIH units/mg protein
Purity (SDS-PAGE)	> 90% (Single chain & α-thrombin)
Assay (HPLC)	> 95%
Solubility	Clear, colorless solution in water or buffer
pH (1% Solution)	6.5 - 7.5
Loss on Drying	< 5.0 %
Bacterial Endotoxins	< 0.1 EU/ NIH unit

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.