Description

Pyruvate Oxidase CAS NO 9001-96-1 is a highly specific oxidoreductase enzyme that catalyzes the oxidative decarboxylation of pyruvate, a critical reaction in metabolic pathways. This enzyme is essential for biochemical research, diagnostic assay development, and industrial biocatalysis, offering precise and efficient substrate conversion. It is a key reagent for manufacturers and researchers in the pharmaceutical, diagnostic, food science, and life science research sectors.

Application

• Diagnostic Kits & Biosensors: Core component in enzymatic assays for the clinical and point-of-care detection of pyruvate, phosphate, or oxygen levels.
• Biochemical Research: Used in metabolic pathway studies, enzyme kinetics experiments, and as a model enzyme for oxidation-reduction research.
• Food & Beverage Analysis: Employed in enzymatic methods for quality control, such as determining pyruvate content in fermented products like wine, beer, and dairy.
• Pharmaceutical Intermediates: Acts as a biocatalyst in the synthesis of fine chemicals and chiral intermediates for drug development.
• Industrial Biocatalysis: Utilized in bio-based production processes and bioremediation applications requiring specific oxidative reactions.
• Educational Kits: Supplied for laboratory teaching modules in university and college biochemistry courses.

Basic Information

Product Name	Pyruvate Oxidase
CAS No.	9001-96-1
Molecular Formula	N/A (Enzyme)
Molecular Weight	~260 kDa (Tetramer, species-dependent)
Synonyms	Pyruvate Dehydrogenase (Oxidase); Pyruvate:Oxygen 2-Oxidoreductase (Phosphorylating); POX; Pyruvate Oxidase (E.C. 1.2.3.3); Pyruvic Oxidase; Pyruvate Dehydrogenase Complex Component E1; Thiamine Pyrophosphate-dependent Pyruvate Dehydrogenase
EINECS	232-656-6

Quality Control

Our Pyruvate Oxidase is produced and tested under stringent quality management systems. Each batch is characterized for specific activity, purity, and stability to ensure consistent performance for critical applications. A comprehensive Certificate of Analysis (COA) detailing assay results, including activity units (U/mg), protein content, and impurity profiles, is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For in-use stability, keep the prepared solution on ice and use promptly.

Specification

Item	Specification
Appearance	Lyophilized powder or suspension
Identification (Activity)	Conforms (Oxidation of Pyruvate)
Specific Activity	≥ 10 U/mg protein
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	≥ 90%
Stabilizers	May contain phosphate buffer, TPP, FAD
pH (in solution)	6.0 - 7.5

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.