Description

Ec 3.1.4.4 Type Iv is a highly purified phospholipase D enzyme preparation, specifically the Type IV isoform, with the CAS registry number 9001-87-0. This enzyme is critical for catalyzing the hydrolysis of phospholipids, enabling precise modification of lipid structures in advanced biochemical processes. It is an essential reagent for research and industrial applications in biotechnology, pharmaceutical development, and specialty chemical synthesis where controlled phospholipid transformation is required.

Application

• Phospholipid Modification & Synthesis: Key catalyst for the head-group exchange of phospholipids (transphosphatidylation) to produce specialized phospholipids like phosphatidylserine (PS) or phosphatidylglycerol (PG).
• Biocatalysis in Fine Chemicals: Used in the enzymatic synthesis of high-value compounds for the nutraceutical and cosmetic industries.
• Pharmaceutical Research & Development: Employed in drug delivery system development, particularly for liposome surface engineering and membrane protein studies.
• Food Ingredient Processing: Applied in the modification of lecithin to improve its emulsifying properties or create novel functional ingredients.
• Diagnostic Reagent Manufacturing: A component in enzymatic assay kits for clinical and research diagnostics targeting lipid metabolism.
• Biochemical Research: Essential tool for studying cell membrane dynamics, signal transduction pathways, and lipid second messengers in academic and industrial labs.

Basic Information

Product Name	Ec 3.1.4.4 Type Iv
CAS No.	9001-87-0
Molecular Formula	Protein (Enzyme)
Molecular Weight	Varies by isoform and source (typically ~90-120 kDa)
Synonyms	Phospholipase D (Type IV); PLD; Lecithinase D; Phosphatidylcholine phosphatidohydrolase; Choline phosphatase; 1,2-Diacyl-sn-glycero-3-phosphocholine phosphatidohydrolase; PLD1; Streptomyces chromofuscus PLD (common source); Cabbage PLD
EINECS	232-647-1

Quality Control

Our Ec 3.1.4.4 Type Iv is produced and tested under strict quality management systems. Each batch is characterized for specific activity, purity, and absence of contaminating enzymatic activities. A comprehensive Certificate of Analysis (COA) is provided, detailing parameters such as protein content, specific activity (U/mg), pH optimum, temperature stability, and purity assessed by SDS-PAGE. We support compliance with relevant guidelines for research and process application.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For short-term use (up to one week), aliquots may be stored at 2-8°C.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	> X U/mg protein (Lot-specific)
Protein Content (Biuret)	> 90%
Purity (SDS-PAGE)	Single major band
pH Optimum	5.5 - 8.0 (Lot-specific)
Solubility	Soluble in buffer or water
Microbial Limits	< 100 CFU/g

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.