Description

3-Phosphoglyceric Phosphokinase is a crucial enzyme in the glycolytic pathway, catalyzing the transfer of a phosphate group from 1,3-bisphosphoglycerate to ADP, forming ATP and 3-phosphoglycerate. This reaction is fundamental to cellular energy metabolism, making the enzyme an essential reagent for biochemical research and diagnostic applications. It is primarily utilized by researchers in biochemistry and molecular biology, as well as by manufacturers of in-vitro diagnostic kits for clinical analysis.

Application

• Biochemical Research: A key reagent for studying glycolysis, gluconeogenesis, and cellular energy metabolism in vitro.
• Diagnostic Kit Manufacturing: Used as a critical component in enzymatic assay kits for clinical diagnostics, such as those measuring ATP, ADP, or related metabolites.
• Enzyme Kinetics Studies: Employed to investigate enzyme mechanisms, inhibition, and catalytic efficiency due to its well-defined role.
• Metabolic Pathway Analysis: Essential for reconstructing and analyzing glycolytic and gluconeogenic pathways in cell-free systems.
• Pharmaceutical Development: Used in screening assays to identify potential modulators of glycolytic activity for therapeutic applications.
• Educational Purposes: Serves as a standard enzyme for teaching advanced concepts in biochemistry and enzymology laboratories.

Basic Information

Product Name	3-Phosphoglyceric Phosphokinase
CAS No.	9001-83-6
Molecular Formula	N/A (Protein)
Molecular Weight	~90-100 kDa (Tetramer)
Synonyms	Phosphoglycerate Kinase; PGK; 3-Phosphoglycerate Kinase; ATP:3-Phospho-D-glycerate 1-Phosphotransferase; EC 2.7.2.3; 1,3-Diphosphoglycerate Kinase; Glycerate Phosphokinase
EINECS	Contact for details

Quality Control

Our 3-Phosphoglyceric Phosphokinase is produced and tested under stringent quality management systems. Each lot is characterized for specific activity, purity, and stability to ensure consistent performance in sensitive applications. A comprehensive Certificate of Analysis (COA) detailing assay results, protein content, and specific activity is provided with every shipment, supporting compliance with research and diagnostic-grade requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation. Aliquotting is recommended for repeated use to avoid freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or suspension
Identification (Activity)	Conforms
Specific Activity	≥ 350 units/mg protein
Protein Content (Biuret)	≥ 90%
Purity (SDS-PAGE)	≥ 95%
pH (in solution)	6.0 - 7.5
Stabilizers	May contain glycerol or salts (see COA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.