Description

Alkaline Phosphatase CAS NO 9001-78-9 is a hydrolase enzyme that catalyzes the dephosphorylation of a wide variety of molecules, including proteins, nucleotides, and alkaloids, in an alkaline pH environment. This enzymatic activity is critical for numerous diagnostic, research, and industrial processes requiring precise phosphate group removal. It is an essential reagent for biotechnology, pharmaceutical development, and clinical diagnostics, serving professionals who demand high purity and consistent performance.

Application

• Clinical Diagnostics & ELISA Kits: Widely used as a conjugate in enzyme-linked immunosorbent assays (ELISA) for the detection of antibodies, antigens, and hormones.
• Molecular Biology Research: Employed in nucleic acid dephosphorylation to prevent self-ligation of vectors in cloning procedures (e.g., CIP treatment of DNA).
• Pharmaceutical Manufacturing: Utilized in the synthesis and modification of active pharmaceutical ingredients (APIs) and in quality control testing.
• Food Safety & Quality Testing: Applied in biosensors and assay systems to detect specific contaminants or to monitor food processing.
• Biochemical Reagent Production: A key component in the formulation of buffers, substrates (e.g., for BCIP/NBT), and standardized test kits.
• Immunohistochemistry (IHC): Acts as a label for antibodies in tissue staining to visualize antigen location.

Basic Information

Product Name	Alkaline Phosphatase
CAS No.	9001-78-9
Molecular Formula	N/A (Enzyme)
Molecular Weight	~140,000 Da (Varies by source)
Synonyms	ALP; AlkP; Phosphatase, Alkaline; Orthophosphoric-monoester phosphohydrolase (alkaline optimum); 3.1.3.1; Alkaline Phosphomonoesterase; Intestinal Alkaline Phosphatase; Calf Intestinal Alkaline Phosphatase (CIAP); Bacterial Alkaline Phosphatase (BAP); Shrimp Alkaline Phosphatase (SAP)
EINECS	232-631-4

Quality Control

Our Alkaline Phosphatase is produced and tested under a stringent quality management system. Each lot is characterized for critical parameters including specific activity, purity (via SDS-PAGE and HPLC), and absence of contaminating nucleases. We provide comprehensive Certificates of Analysis (COA) detailing assay results, ensuring the product meets the rigorous standards required for diagnostic and research applications. Specifications can be aligned with relevant pharmacopeial guidelines (e.g., USP) upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at 2-8°C. The product is hygroscopic (moisture-sensitive); ensure the container is kept sealed in a dry environment to maintain activity. Avoid repeated freeze-thaw cycles of prepared solutions.

Specification

Item	Specification
Appearance	Lyophilized powder or suspension
Identification (Activity)	Positive enzymatic activity assay
Specific Activity	≥ [Varies by grade] U/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Content (Biuret)	Reported on COA
DNase/RNase Activity	Not detected
pH (in solution)	7.5 - 8.5
Loss on Drying	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.