Description

Ec 1.1.1.37 CAS NO 9001-64-3 is a high-purity enzyme preparation, specifically Malate Dehydrogenase (MDH), which is critical for catalyzing the reversible oxidation of malate to oxaloacetate in the citric acid cycle. This biocatalyst is essential for research and industrial processes requiring precise metabolic pathway analysis or NAD(P)H regeneration. It is primarily utilized by biotechnology firms, diagnostic kit manufacturers, and academic research institutions focused on enzymology, metabolic engineering, and bioanalytical assay development.

Application

• Diagnostic Reagent Manufacturing: A key component in clinical chemistry test kits for the enzymatic determination of metabolites like malate, oxaloacetate, or NAD(P)H in serum and other biological samples.
• Biochemical Research & Assay Development: Used as a standard enzyme in research laboratories for studying the Krebs cycle, enzyme kinetics, inhibitor screening, and metabolic pathway reconstitution.
• NAD(P)H Cofactor Regeneration Systems: Employed in coupled enzyme assays and biocatalytic synthesis to continuously regenerate the reduced cofactor NAD(P)H, driving other enzymatic reactions.
• Food & Beverage Analysis: Applied in enzymatic methods for the quantitative analysis of specific organic acids (e.g., L-malic acid) in food products, juices, and wines to assess quality and authenticity.
• Pharmaceutical Intermediate Synthesis: Serves as a biocatalyst in the stereoselective synthesis of chiral intermediates for active pharmaceutical ingredients (APIs).
• Educational Kits: Supplied in purified form for university-level teaching kits to demonstrate fundamental principles of enzymology and bioenergetics.

Basic Information

Product Name	Ec 1.1.1.37 (Malate Dehydrogenase)
CAS No.	9001-64-3
Molecular Formula	Protein (Enzyme)
Molecular Weight	~70 kDa (Subunit dependent; contact for details)
Synonyms	Malate Dehydrogenase; MDH; L-Malate:NAD+ Oxidoreductase; EC 1.1.1.37; Malic Dehydrogenase; NAD-dependent Malate Dehydrogenase; Malate NAD Oxidoreductase; Dehydrogenase, Malate
EINECS	232-622-4

Quality Control

Our Malate Dehydrogenase is produced and tested under stringent quality management systems. Each lot is characterized for specific activity, purity, and stability to ensure reliable performance in sensitive applications. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing parameters such as activity units/mg protein, protein concentration, and absence of contaminating enzyme activities. We adhere to relevant guidelines for biochemical reagent quality.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent moisture absorption, which can affect activity. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms (Oxidizes L-Malate)
Specific Activity	≥ 300 units/mg protein
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	≥ 90%
Solubility	Clear solution in buffer
Stabilizers	May contain buffer salts (e.g., phosphate)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.