Description

Isocitrate Dehydrogenase CAS NO 9001-58-5 is a critical enzyme that catalyzes the oxidative decarboxylation of isocitrate to α-ketoglutarate in the Krebs cycle, playing a pivotal role in cellular energy metabolism and biosynthesis. Its reliable activity and purity are essential for accurate biochemical research and diagnostic applications. This high-quality enzyme is indispensable for researchers and manufacturers in the life sciences, pharmaceutical development, and clinical diagnostics sectors.

Application

• Biochemical Research & Assay Development: Used as a key reagent in enzymatic studies, metabolic pathway analysis, and the development of coupled enzyme assays.
• Clinical Diagnostics: Employed in vitro diagnostic (IVD) kits for the measurement of substrates like NADP+ or isocitrate, aiding in disease research and biomarker detection.
• Pharmaceutical Screening: Serves as a target enzyme in high-throughput screening (HTS) platforms for identifying potential inhibitors or activators relevant to metabolic diseases and cancer.
• Educational & Teaching Kits: A fundamental component in laboratory educational kits for demonstrating enzyme kinetics and central metabolic pathways.
• Food & Beverage Analysis: Utilized in enzymatic methods for the specific quantification of organic acids or related compounds in quality control laboratories.
• Biocatalysis: Potential use in specialized synthetic pathways for the production of chiral intermediates or fine chemicals.

Basic Information

Product Name	Isocitrate Dehydrogenase
CAS No.	9001-58-5
Molecular Formula	N/A (Enzyme)
Molecular Weight	Varies by source and subunit composition
Synonyms	IDH; Isocitric Dehydrogenase; Oxalosuccinate Decarboxylase; NADP+-linked Isocitrate Dehydrogenase; EC 1.1.1.42; Three-DS-Isocitrate:NADP+ Oxidoreductase (Decarboxylating)
EINECS	Contact for details

Quality Control

Our Isocitrate Dehydrogenase is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and optimal enzymatic activity. Each lot is characterized for specific activity, purity, and stability. A comprehensive Certificate of Analysis (COA) detailing assay results, protein content, and storage stability is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation. Aliquot reconstituted solutions to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Specific Activity	≥ [Value] units/mg protein
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	≥ 90%
Identification (Activity)	Conforms
pH (in solution)	7.0 - 7.5
Stabilizers/Additives	May contain buffer salts (e.g., phosphate) and stabilizers (e.g., glycerol)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.