Description

Phosphoglucose Isomerase (PGI), also known as Glucose-6-phosphate Isomerase, is a critical enzyme in the glycolytic and gluconeogenic pathways, catalyzing the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Its precise catalytic activity is essential for fundamental energy metabolism, making it a vital reagent for biochemical research, diagnostic applications, and industrial biocatalysis. This high-purity enzyme is primarily utilized by biotechnology firms, diagnostic kit manufacturers, and research institutions in life sciences.

Application

• Diagnostic Reagent Manufacturing: A key component in clinical diagnostic kits for measuring glucose, fructose, and related metabolites in blood and other biological samples.
• Biochemical Research & Assay Development: Used as a standard enzyme in metabolic pathway studies, enzyme kinetics experiments, and the development of coupled enzyme assays.
• Industrial Biocatalysis: Employed in the synthesis of rare sugars and sugar phosphates for the food, nutraceutical, and pharmaceutical industries.
• Pharmaceutical Intermediate Production: Facilitates specific isomerization steps in the synthesis of complex carbohydrate-based active pharmaceutical ingredients (APIs).
• Food Science & Analysis: Applied in enzymatic methods for the accurate quantification of sugar profiles in food and beverage products.
• Educational & Laboratory Use: Serves as a teaching tool and standard reagent in academic and industrial laboratories for biochemistry practicals.

Basic Information

Product Name	Phosphoglucose Isomerase
CAS No.	9001-41-6
Molecular Formula	N/A (Protein)
Molecular Weight	~63 kDa (monomer, varies by source)
Synonyms	Glucose-6-phosphate Isomerase; GPI; Phosphohexose Isomerase; Autocrine Motility Factor (AMF); Neuroleukin (NLK); D-Glucose-6-phosphate ketol-isomerase; EC 5.3.1.9; PGI Enzyme
EINECS	232-619-1

Quality Control

Our Phosphoglucose Isomerase is produced and tested under a stringent quality management system. Each lot is characterized for critical parameters including specific activity, purity, and stability to ensure reproducible performance in sensitive applications. We provide comprehensive Certificates of Analysis (COA) detailing assay results, and our quality systems support compliance with relevant standards for research and in-vitro diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or solution
Identification (Activity)	Conforms (Specific activity ≥ 300 units/mg protein)
Assay (Specific Activity)	300 - 600 units/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Content (Biuret)	Contact for details
pH (in solution)	6.0 - 7.5
Stability	Retains ≥ 90% activity after 12 months at -20°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.