Description

Diaphorase is a vital oxidoreductase enzyme that catalyzes the transfer of electrons from NADH or NADPH to various electron acceptors, including ferricyanide and cytochrome c. This catalytic activity makes it an indispensable component in diagnostic and research applications, particularly in enzymatic cycling assays and biosensor systems. It is essential for industries focused on clinical diagnostics, biochemical research, and the development of analytical reagents.

Application

• Clinical Diagnostic Kits: A key component in enzymatic assays for measuring substrates like glucose, lactate, and cholesterol, enabling accurate medical diagnostics.
• Biochemical Research Reagents: Used as a coupling enzyme in dehydrogenase-linked assays to amplify signals and improve detection sensitivity in research laboratories.
• Biosensor Development: Integrated into electrode surfaces for the creation of amperometric biosensors, facilitating rapid and specific detection of analytes.
• NAD(P)H Regeneration Systems: Employed in biocatalysis and synthetic biology to efficiently recycle expensive cofactors like NADH or NADPH.
• Food Safety Testing: Utilized in enzymatic methods for the detection of contaminants or specific compounds in food and beverage quality control.
• Pharmaceutical Analysis: Supports drug discovery and quality control processes through its use in high-throughput screening assays and stability testing.

Basic Information

Product Name	Diaphorase
CAS No.	9001-18-7
Molecular Formula	N/A (Enzyme)
Molecular Weight	Varies by source (typically ~30-50 kDa)
Synonyms	Diaphorase (NADH); NADH Dehydrogenase; NADH-Cytochrome b5 Reductase; Lipoamide Dehydrogenase (from some sources); Dihydrolipoamide Dehydrogenase; EC 1.6.99.-; EC 1.8.1.4; Heart Diaphorase
EINECS	Contact for details

Quality Control

Our Diaphorase is produced and tested under stringent quality management systems. Each batch is characterized for critical parameters including specific activity, purity, and stability to ensure consistent performance in sensitive applications. A comprehensive Certificate of Analysis (COA), detailing assay results, is provided with every shipment to guarantee compliance with your technical requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent moisture absorption, which can affect activity. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	≥ [Value] units/mg protein
Protein Content (Biuret)	≥ [Value] %
Purity (SDS-PAGE)	≥ [Value] %
Solubility	Clear solution at 10 mg/mL in buffer
pH (Solution)	6.0 - 8.0
Loss on Drying	≤ [Value] %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.