Description

Butyrylcholinesterase is a crucial serine hydrolase enzyme that catalyzes the hydrolysis of choline esters, including butyrylcholine and other neuroactive compounds. Its primary commercial and research value lies in its role as a biomarker and a key reagent for toxicological and pharmacological studies, particularly in the assessment of exposure to organophosphate and carbamate pesticides or nerve agents. This enzyme is essential for diagnostic kit manufacturers, academic research institutions, and pharmaceutical companies engaged in neuroscience, toxicology, and drug discovery.

Application

• Diagnostic Reagent: Core component in clinical diagnostic kits for measuring liver function and for the detection of organophosphate poisoning.
• Toxicology & Environmental Testing: Used as a standard reagent in assays to screen for and quantify exposure to cholinesterase-inhibiting pesticides and chemical warfare agents.
• Pharmacological Research: Employed in high-throughput screening (HTS) to identify and evaluate potential new drugs for Alzheimer's disease, myasthenia gravis, and other neurological conditions.
• Biochemical Research: Serves as a model enzyme for studying enzyme kinetics, inhibition mechanisms, and protein structure-function relationships.
• Biomarker Studies: Utilized in research investigating its role as a biomarker for liver disease, inflammation, and metabolic syndromes.
• Food Safety Analysis: Applied in biosensor development for the rapid detection of pesticide residues on agricultural products.

Basic Information

Product Name	Butyrylcholinesterase
CAS No.	9001-08-5
Molecular Formula	N/A (Protein)
Molecular Weight	Approx. 340 kDa (Tetramer)
Synonyms	BChE; Pseudocholinesterase; Plasma Cholinesterase; Acylcholine Acylhydrolase; Butyrylcholine Esterase; Cholinesterase II; Non-specific cholinesterase; Benzoylcholinesterase; Choline esterase II
EINECS	232-625-4

Quality Control

Our Butyrylcholinesterase is produced and tested under a rigorous quality management system. Each batch is characterized for specific activity, purity, and stability to ensure consistent performance in sensitive applications. Certificates of Analysis (COA) detailing parameters such as activity (U/mg), protein content, and inhibitor sensitivity are provided to guarantee compliance with research and diagnostic standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C. Reconstituted enzyme solutions should be aliquoted and stored at -20°C or below to prevent repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Activity)	Conforms (Hydrolyzes butyrylthiocholine)
Specific Activity	≥ 10 U/mg protein (Contact for specific grade)
Purity (SDS-PAGE)	≥ 90%
Protein Content (Biuret)	≥ 90% of dry weight
Solubility	Clear, colorless solution in buffer
Loss on Drying	≤ 5.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.