Description

D-Amino Acid Oxidase is a highly specific flavoenzyme that catalyzes the oxidative deamination of D-amino acids, producing the corresponding α-keto acids, ammonia, and hydrogen peroxide. This enzymatic activity is critical for research and industrial processes requiring the selective metabolism or detection of D-amino acid enantiomers. It is an essential reagent for pharmaceutical development, biochemical research, and the production of fine chemicals and chiral intermediates.

Application

• Biocatalysis & Chiral Synthesis: Used in the production of enantiomerically pure L-amino acids and other chiral building blocks for pharmaceuticals and agrochemicals.
• Diagnostic Kits & Biosensors: Serves as a key component in enzymatic assays and biosensor systems for the quantitative detection of D-amino acids, relevant in clinical and food analysis.
• Pharmaceutical Research: Employed in drug discovery and metabolic pathway studies, particularly in neuroscience research related to D-serine and N-methyl-D-aspartate (NMDA) receptor function.
• Food & Beverage Analysis: Applied in quality control to measure D-amino acid content, which can indicate fermentation processes, aging, or adulteration.
• Enzyme Engineering Studies: Used as a model enzyme in protein engineering and stability studies due to its well-characterized structure and mechanism.
• Industrial Bioprocessing: Integrated into bioreactor systems for the continuous removal or conversion of D-amino acids in complex fermentation broths.

Basic Information

Product Name	D-Amino Acid Oxidase
CAS No.	9000-88-8
Molecular Formula	N/A (Protein)
Molecular Weight	~39 kDa (Monomer, species-dependent)
Synonyms	DAAO; D-Amino-Acid Oxidase; D-Aspartate Oxidase; D-Amino Acid:Oxygen Oxidoreductase (Deaminating); EC 1.4.3.3; R-α-Amino-acid Oxidase; D-AA Oxidase
EINECS	Contact for details

Quality Control

Our D-Amino Acid Oxidase is produced and tested under stringent quality management systems. Each batch is characterized for specific activity, purity, and stability to ensure consistent performance. A comprehensive Certificate of Analysis (COA) is provided, detailing parameters such as activity (U/mg protein), protein content, and absence of contaminating protease activities. We support compliance with relevant guidelines for research and industrial use.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C. Reconstituted enzyme solutions should be aliquoted and stored at -20°C or below to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use in a low-humidity environment.

Specification

Item	Specification
Appearance	Lyophilized powder or suspension
Specific Activity	≥ 10 U/mg protein
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	≥ 90%
Identification (Activity)	Conforms
pH Optimum	~8.5
Stability	Stable for 24 months at -20°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.