Description

2-[(5-Fluoresceinyl)Aminocarbonyl]Ethyl Methanethiosulfonate is a specialized, high-purity chemical reagent designed for advanced bioconjugation and labeling applications. This compound is critical for researchers and developers requiring precise, site-specific modification of biomolecules, particularly proteins and peptides, with a fluorescent tag. It is primarily utilized in the life sciences, pharmaceutical R&D, and diagnostic assay development sectors for creating sensitive probes and studying molecular interactions.

Application

• Site-Specific Protein Labeling: Used as a thiol-reactive probe for the selective fluorescent labeling of cysteine residues in proteins and antibodies.
• Bioconjugation Reagent: A key intermediate in the synthesis of fluorescently tagged biomolecules for use in assays and diagnostic kits.
• Fluorescence Resonance Energy Transfer (FRET) Probes: Enables the construction of FRET-based molecular beacons and sensors for real-time monitoring of enzymatic activity or binding events.
• Cell Biology & Imaging: Facilitates the labeling of intracellular targets for fluorescence microscopy and flow cytometry analysis.
• Diagnostic Development: Employed in the manufacturing of fluorescent conjugates for immunoassays (e.g., ELISA, lateral flow tests) and in vitro diagnostics (IVD).
• Drug Discovery: Used in high-throughput screening (HTS) platforms to label target proteins and monitor ligand binding or protein-protein interactions.

Basic Information

Product Name	2-[(5-Fluoresceinyl)Aminocarbonyl]Ethyl Methanethiosulfonate
CAS No.	887355-30-8
Molecular Formula	C25H19NO8S2
Molecular Weight	525.55 g/mol
Synonyms	Fluorescein-5-maleimide thiosulfonate; 5-Fluorescein maleimide thiosulfonate; Fluorescein maleimide thiosulfonate; MTS-Fluorescein; 2-[(5-Fluoresceinyl)Aminocarbonyl]Ethyl Methanethiosulfonate; 5-FAM maleimide thiosulfonate; Fluorescein-based MTS reagent; Thiol-reactive fluorescein probe
EINECS	Contact for details

Quality Control

Every batch of 2-[(5-Fluoresceinyl)Aminocarbonyl]Ethyl Methanethiosulfonate is manufactured and tested under strict quality management systems to ensure the highest standards of purity and performance for research and diagnostic applications. Our products undergo rigorous analytical testing, including HPLC for purity and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing batch-specific results is provided with each shipment to guarantee traceability and compliance with your project requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This material is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red solid
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.