Description

5-Bromo-6-Chloro-3-Indolyl α-D-Glucopyranoside is a specialized chromogenic substrate used in enzymatic detection and diagnostic assays. This compound is critical for researchers and manufacturers requiring high-purity, reliable reagents for sensitive biochemical applications. It is primarily utilized in the life sciences, pharmaceutical research, and in-vitro diagnostic (IVD) kit production for the detection of specific glycosidase enzymes.

Application

• Chromogenic Substrate for Glycosidases: Used as a key reagent in enzymatic assays to detect and quantify α-glucosidase activity, where cleavage yields an insoluble, colored indigo derivative.
• Microbiological Research: Employed in microbial identification and characterization studies based on specific enzymatic profiles.
• Diagnostic Kit Manufacturing: A vital component in the formulation of commercial in-vitro diagnostic (IVD) kits for clinical laboratories.
• Biochemical Research: Utilized in enzyme kinetics studies, inhibitor screening, and pathway analysis in academic and industrial R&D settings.
• Histochemistry & Cell Biology: Applied in staining techniques to localize enzyme activity within tissues and cells.
• Quality Control in Bioprocessing: Used to monitor enzyme activity and purity in biomanufacturing processes.

Basic Information

Product Name	5-Bromo-6-Chloro-3-Indolyl α-D-Glucopyranoside
CAS No.	878495-64-8
Molecular Formula	C14H15BrClNO6
Molecular Weight	408.63 g/mol
Synonyms	5-Bromo-6-chloro-3-indolyl-α-D-glucopyranoside; X-α-Glc; 5-Br-6-Cl-3-Indolyl-α-D-glucoside; BCIP-α-Glc; α-D-Glucopyranoside, 5-bromo-6-chloro-3-indolyl; Chromogenic α-glucosidase substrate; 878495-64-8
EINECS	Contact for details

Quality Control

Our 5-Bromo-6-Chloro-3-Indolyl α-D-Glucopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot is subjected to rigorous analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; sparingly soluble in water
Heavy Metals	≤ 20 ppm
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.