Description

n-(2-Hydroxyethyl)Piperazine-n-2-Hydroxypropanesulfonic Acid Hydrate (Heppso) is a high-purity zwitterionic biological buffer agent, also known as HEPPSO. This compound is valued for its excellent buffering capacity in the physiological pH range, providing superior stability for sensitive biochemical systems. It is primarily used by researchers and manufacturers in biotechnology, pharmaceutical development, and diagnostic kit formulation where precise pH control is critical. The hydrate form ensures consistent handling and solubility for reproducible experimental and production outcomes.

Application

• Biochemical Research & Cell Culture: As a key component in buffer systems for enzyme assays, protein purification, and mammalian cell culture media to maintain optimal pH.
• Molecular Biology: Used in electrophoresis buffers (e.g., for capillary electrophoresis) and nucleic acid hybridization solutions to prevent pH drift.
• Diagnostic Reagents: Formulation of stable buffer matrices for clinical diagnostic assays and immunoassays requiring consistent ionic strength.
• Pharmaceutical Manufacturing: Serves as an excipient or process buffer in the production of biologics and other pH-sensitive drug formulations.
• Cosmetic & Personal Care: Acts as a pH adjuster and stabilizer in high-performance skincare and cosmetic products.

Basic Information

Product Name	n-(2-Hydroxyethyl)Piperazine-n-2-Hydroxypropanesulfonic Acid Hydrate (Heppso)
CAS No.	865856-46-8
Molecular Formula	C9H20N2O5S • xH2O
Molecular Weight	268.33 g/mol (anhydrous basis)
Synonyms	HEPPSO; HEPPSO Hydrate; 3-[4-(2-Hydroxyethyl)piperazin-1-yl]-2-hydroxypropanesulfonic acid hydrate; 2-Hydroxy-3-[4-(2-hydroxyethyl)-1-piperazinyl]propanesulfonic acid hydrate; EPPS Hydrate; Good's Buffer HEPPSO; N-(2-Hydroxyethyl)piperazine-N'-(2-hydroxypropanesulfonic acid) hydrate
EINECS	Contact for details

Quality Control

Our n-(2-Hydroxyethyl)Piperazine-n-2-Hydroxypropanesulfonic Acid Hydrate (Heppso) is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity, titration for assay, and stringent tests for heavy metals and residual solvents to ensure it meets the high standards required for life science applications. Certificates of Analysis (COA) with full traceability are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, well-ventilated area at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container must be kept tightly sealed after use to prevent moisture absorption.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (Titration)	≥ 98.5%
pH (1% Solution)	7.5 - 8.5
Loss on Drying	≤ 2.0%
Heavy Metals (as Pb)	≤ 10 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.