Description

Bis(2,5-Dioxopyrrolidin-1-Yl) 2,2'-Disulfanediyldiacetate is a specialized heterobifunctional crosslinking agent featuring an activated ester and a disulfide bond. This compound is critical for creating reversible, cleavable linkages in bioconjugation and materials science, offering precise control over molecular assembly. It is primarily utilized by researchers and manufacturers in pharmaceutical development, proteomics, and advanced polymer synthesis who require site-specific and reducible conjugation strategies.

Application

• Bioconjugation Reagent: For the site-specific crosslinking of proteins, antibodies, or peptides to other biomolecules, nanoparticles, or solid surfaces via amine groups, with the disulfide bond allowing for cleavage under reducing conditions.
• ADC (Antibody-Drug Conjugate) Linker Development: Serves as a key intermediate in the research and synthesis of cleavable linkers for targeted drug delivery systems.
• Polymer Functionalization: Used to introduce reactive NHS-ester groups and disulfide bridges into polymer backbones for creating smart, stimuli-responsive materials.
• Surface Modification: Enables the controlled immobilization of biomolecules onto chips, beads, or other substrates for biosensor and diagnostic assay development.
• Proteomics Research: Employed in crosslinking mass spectrometry (XL-MS) studies to investigate protein-protein interactions and structural dynamics.
• Hydrogel Formation: Acts as a crosslinker for fabricating hydrogels with tunable mechanical properties and degradation profiles via disulfide reduction.

Basic Information

Product Name	Bis(2,5-Dioxopyrrolidin-1-Yl) 2,2'-Disulfanediyldiacetate
CAS No.	863649-56-3
Molecular Formula	C₁₄H₁₄N₂O₈S₂
Molecular Weight	410.40 g/mol
Synonyms	NHS-SS-NHS; Disuccinimidyl 3,3'-dithiodipropionate; DSP Crosslinker; DTSSP (3,3'-Dithiobis(sulfosuccinimidyl propionate)) analog; Bis(N-succinimidyl) 3,3'-dithiodipropionate; 2,2'-Dithiobis(acetic acid) bis(2,5-dioxopyrrolidin-1-yl) ester; 1,1'-[Dithiobis(2,1-ethanediyloxycarbonyl-2,1-ethanediyl)]bis-2,5-pyrrolidinedione
EINECS	Contact for details

Quality Control

Our Bis(2,5-Dioxopyrrolidin-1-Yl) 2,2'-Disulfanediyldiacetate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment and NMR for structural confirmation, to ensure high batch-to-batch consistency and compliance with research-grade specifications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.